| 严婉荣 1
，赵志祥 1
，肖彤斌 1
，张善学 2，文香玲 2，王会芳 1.海南辣椒、樱桃番茄病毒病调查
及病原 dot-ELISA 鉴定[J].广东农业科学,2021,48(1):119-125 |
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海南辣椒、樱桃番茄病毒病调查
及病原 dot-ELISA 鉴定 |
| Investigation and dot-ELISA Pathogen Identification of Virus Diseases in Pepper and Cherry Tomato in Hainan |
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| DOI：10.16768/j.issn.1004-874X.2020.07.015 |
| 中文关键词: 病毒病症状 发病率 病情指数 病毒检出率 优势毒源 复合侵染 |
| 英文关键词: viral disease symptom incidence rate disease index virus detection rate dominant virus source compound
infection |
| 基金项目:海南省重大科技计划项目（zdkj201813）；海南省重点研发计划项目（ZDYF2018237） |
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| 中文摘要: |
| 【目的】调查和鉴定海南省辣椒和樱桃番茄病毒病发生情况和病毒种类，明确病毒病发生为害程
度及毒源种类。【方法】2018—2019 年，通过 5 点取样法对海南省 10 个市县的辣椒和樱桃番茄进行病毒症状类
型、发病率和病情指数统计。采集辣椒和樱桃番茄主产区病毒病样本 593 份，通过斑点酶联免疫吸附法（dotELISA）对黄瓜花叶病毒（Cucumber mosaic virus, CMV）、烟草花叶病毒（ Tobacco mosaic virus,TMV）、芜菁花
叶病毒（Turnip mosaic virus,TuMV）、蚕豆萎蔫病毒（Broad bean wilt virus,BBWV）和黄瓜绿斑驳病毒（Cucumber
green mottle mosaic virus,CGMMV）5 种病毒进行检测。【结果】辣椒病毒病主要症状是斑驳花叶和矮缩丛枝，发
病率在 0~75% 之间，病情指数在 0~25.67 之间。樱桃番茄主要症状是皱缩卷曲和斑驳花叶，发病率在 7%~98%
之间，病情指数在 1.67~37.33 之间。辣椒病毒总体检出率为 37.5%，复合侵染率为 2.08%，樱桃番茄病毒总体检
出率为 20.33%，复合侵染率为 0.98%。【结论】病毒病在辣椒和樱桃番茄上普遍发生，研究结果明确了辣椒和
樱桃番茄田间发病状况，dot-ELISA 检测结果表明 CMV 和 TMV 仍是检测的 5 种病毒中的优势毒源，存在 4 种类
型的复合侵染。 |
| 英文摘要: |
| 【Objective】The study was conducted to investigate and identify the occurrence of virus diseases and virus
types of peppers and cherry tomatoes in Hainan Province, and to determine the degree of damage and source of virus.【Method】
From 2018 to 2019, the symptoms, incidence rates and disease indexes of the viruses were analyzed for peppers and cherry
tomatoes in 10 cities and counties of Hainan Province by 5 point sampling method. A total of 593 virus samples were collected
from the main production areas of pepper and cherry tomato, to detect cucumber mosaic virus（CMV）, tobacco mosaic virus
（TMV）, turnip mosaic virus （TuMV）, broad bean wilt virus （BBWV） and cucumber green mottle mosaic virus （CGMMV）by using the dot enzyme-linked immunosorbent assay （dot-ELISA）.【Result】The main symptoms of capsicum virus disease
were characterized by mottled mosaic leaves and dwarf twigs, the incidence rate was 0-75%, and the disease index was 0-25.67.
The main symptoms of cherry tomatoes were shrinkage and curling with mottled floral leaves. The incidence rate was 7%-98%,
and the disease index was 1.67-37.33. The total detection rate of pepper virus was 37.5%, the compound infection rate was 2.08%,
while the total detection rate of cherry tomato virus was 20.33%, and the compound infection rate was 0.98%.【Conclusion】Virus
diseases are common in peppers and cherry tomatoes. The study results confirmed the disease status of pepper and cherry tomato
in the field. The results of dot-ELISA showed that CMV and TMV were still the dominant virus sources among the five viruses
detected, and there were four types of compound infection. |
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