施钼对黑果枸杞幼苗生长及荧光参数的影响

聂必林; 田中平; 巫利梅; 如马南木·尼合买提; 吕海英

文章摘要

聂必林，田中平，巫利梅，如马南木·尼合买提，吕海英.施钼对黑果枸杞幼苗生长及荧光参数的影响[J].广东农业科学,2021,48(7):24-31

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Effects of Molybdenum Application on Growth and Fluorescence Parameters of Lycium ruthenicum Seedlings

DOI：10.16768/j.issn.1004-874X.2021.07.004

中文关键词: 黑果枸杞钼肥幼苗生长荧光参数微量元素

英文关键词: Lycium ruthenicum molybdenum fertilizer seedling growth fluorescence parameter trace element

基金项目:新疆维吾尔自治区高校科研计划项目（XJEDU2019Y034）；新疆维吾尔自治区重点实验室招标课题（XJDX1414-2018-03）；新疆师范大学优秀青年教师科研启动基金（XJNU201509）；国家自然科学基金（31560058，31901096）

作者	单位
聂必林，田中平，巫利梅，如马南木·尼合买提，吕海英	1. 新疆师范大学生命科学学院，新疆乌鲁木齐 830053； 2. 干旱区植物逆境生物学实验室，新疆乌鲁木齐 830054； 3. 新疆特殊环境物种保护与调控生物学实验室，新疆乌鲁木齐 830054

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中文摘要:

【目的】研究不同施钼浓度对黑果枸杞幼苗生长及荧光参数的影响，初步确定促进黑果枸杞幼苗生长的最适施钼浓度，为黑果枸杞小浆果产业发展提供一定的基础理论参考。【方法】以黑果枸杞幼苗为研究对象，采用水培种植试验，探究不同施钼浓度下黑果枸杞幼苗根长、株高、生物量、微量元素含量及荧光参数指标变化。【结果】（1）随钼处理浓度升高，黑果枸杞幼苗各部位生物量（鲜重和干重）、根长及株高均呈现先升后降的变化趋势。钼浓度为 5、10、20 μmol/L 时对黑果枸杞幼苗各项生长指标具有显著促进作用，以 10 μmol/L 综合促进效果最佳，钼浓度为 40 μmol/L 时则产生抑制作用。（2）施钼对微量元素 Mo、Zn、Fe 的吸收具有明显影响，对 Mo 元素的吸收利用表现为协同关系，对 Zn 元素表现为拮抗关系，对 Fe 元素表现为拮抗和协同双重关系，施钼浓度在 10 μmol/L 时黑果枸杞幼苗各部位对微量元素 Mo、Zn、Fe 吸收利用效率较为稳定。（3）黑果枸杞幼苗初始荧光（Fo）随施钼浓度升高呈现先降后升的变化趋势，在钼处理浓度为 10 μmol/L 时有最小值。最大荧光产量（Fm）、最大光化学效率（Fv/Fm）及实际光化学效率（Φps Ⅱ）的变化趋势与 Fo 相反，在钼处理浓度为 5 μmol/L 或 10 μmol/L 时有最大值。【结论】黑果枸杞幼苗生长与施钼浓度之间具有明显的相关性，适宜的施钼浓度能显著提高黑果枸杞幼苗的生长，浓度过高又会抑制其生长。适合黑果枸杞幼苗生长的钼浓度范围为 5~20 μmol/L，以 10 μmol/L 最佳。

英文摘要:

【Objective】The study was carried out to explore the effects of different molybdenum（Mo） concentrations on the growth and fluorescence parameters of Lycium ruthenicum seedlings, and to preliminarily determine the optimal Mo concentration to promote the growth of L. ruthenicum seedlings, which provided a certain basic theoretical reference for the development of small berry industry.【Method】Taking L. ruthenicum seedlings as the research object, hydroponic planting experiments were conducted to explore the changes in root length, plant height, biomass, trace elements content and fluorescence parameters of L. ruthenicum seedlings under different Mo concentrations.【Result】（1）With the increase of Mo concentration, the biomass, root length and plant height of all the parts of L. ruthenicum seedlings showed a trend of first increasing and then decreasing. The Mo concentrations of 5, 10 and 20 μmol/L could significantly promote the various growth indicators of L. ruthenicum seedlings, and the Mo concentration of 10 μmol/L had the best comprehensive promotion effect; when the Mo concentration was 40 μmol/L, it had an inhibitory effect;（2）Mo application had a significant effect on the absorption of trace elements Mo, Zn and Fe, and the absorption and utilization of Mo element was synergistic, that of Zn was antagonistic, and that of Fe was antagonistic and synergistic. When Mo concentration was 10 μmol/L, absorption and utilization efficiency of each part in ruthenicum seedlings was relatively stable to trace elements Mo, Zn and Fe;（3） With the increase of Mo concentration, the initial fluorescence（Fo）of L. ruthenicum showed a trend of first decreasing and then increasing, and there was a minimum value when the Mo concentration is 10 μmol/L. The trend of maximum fluorescence yield（Fm）, maximum photochemical efficiency（Fv/Fm）and actual photochemical efficiency（ΦPSⅡ ）was opposite to that of Fo, and there was a maximum when the Mo concentration was 5 μmol/L or 10 μmol/L.【Conclusion】There is an obvious correlation between the growth of L. ruthenicum seedlings and the concentration of Mo applied. Mo application at an appropriate concentration can significantly increase the growth of L. ruthenicum seedlings, and it will inhibit its growth when the concentration is too high. Experiments have found that the suitable Mo concentration range for the growth of L. ruthenicum seedlings is 5 -20 μmol/L, with 10 μmol/L being the best.

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