| 蒋 锋，闫 艳，罗智佳，陈青春，张姿丽，刘鹏飞.甜玉米维生素 C 含量的 QTL 定位[J].广东农业科学,2021,48(11):18-24 |
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甜玉米维生素 C 含量的 QTL 定位 |
| QTL Mapping of Vitamin C Content in Sweet Corn |
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| DOI：10.16768/j.issn.1004-874X.2021.11.003 |
| 中文关键词: 甜玉米 维生素 C 含量 QTL 分子标记选择 SSR 分子标记 连锁图谱 |
| 英文关键词: sweet corn vitamin C content QTL molecular marker selection SSR molecular marker linkage map |
| 基金项目:广东省鲜食玉米遗传育种工程技术研究中心（粤科产学研 [2016]176 号）；广东省重点领域研发计划（2018B020202013）；广州市特色作物种质资源研究与利用重点实验室项目（202002010010） |
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| 中文摘要: |
| 【目的】挖掘利用高维生素甜玉米种质资源，对甜玉米维生素 C 含量进行 QTL 定位。【方法】选用维生素 C 含量差异显著的甜玉米自交系 T77（高 VC）和 T15（低 VC）为亲本配制杂交组合 T77×T15，以该组合 F2 作为群体，构建 SSR 分子标记遗传连锁图谱；结合 F2 各单株果穗维生素 C 含量，应用复合区间作图法对甜玉米维生素 C 含量进行 QTL 定位。【结果】共检测出 5 个相关 QTL，分别位于第 2、3、6 染色体。在第 2染色体 bin 2.04~2.05 区域检测到 1 个维生素 C 含量 QTL（qVC-ch.2-1），加性效应值为 2.23，能够解释 17.16%的表型变异；在第 3 染色体上检测到 2 个维生素 C 含量 QTL（qVC-ch.3-1、qVC-ch.3-2），分别位于 bin 3.00~3.04、bin 3.06~3.07 区域，加性效应值分别为 1.17 和 1.40，表型贡献率分别为 6.64% 和 4.15%；在第 6 染色体上检测到 2 个维生素 C 含量 QTL（qVC-ch.6-1、qVC-ch.6-2），分别位于 bin 6.05、bin 6.05~6.06 区域，加性效应值分别为 0.43、1.83，表型贡献率分别为 6.59% 和 20.87%。【结论】维生素 C 含量的 QTL 定位可为选育优质高营养甜玉米品种提供一定的理论参考。 |
| 英文摘要: |
| 【Objective】In order to exploit and utilize high vitamin sweet corn germplasm resources, QTL mapping of vitamin C content in sweet corn was carried out.【Method】The inbred lines T77 (high VC) and T15 (low VC), which have significant differences in vitamin C content, were used as parents to prepare the hybrid combination T77×T15, and the combination F2 was used as mapping population to construct SSR molecular marker genetic linkage map. Combined with the vitamin C content of each individual ear in F2, QTL mapping of vitamin C content in sweet corn was carried out by using compound interval mapping method.【Result】Five related QTL were detected, which were located on chromosome 2, 3 and 6. One vitamin C QTL (qVC-ch.2-1) was detected in the bin2.04-2.05 region of chromosome 2, with an additive effect value of 2.23, which could explain 17.16% of phenotypic variation. Two QTL for vitamin C content (qvc-ch.3-1 and qVC-ch.3-2) were detected on chromosome 3, which were located in bin3.00-3.04 and bin3.06-3.07 respectively, with additive effect values of 1.17 and 1.40, and phenotypic contribution rates of 6.64% and 4.15% respectively. Two QTL for vitamin C content (qvc-ch.6-1 and qVC-ch.6-2) were detected on chromosome 6, which were located in 6.05 and 6.05-6.06 regions, with additive effects value of 0.43 and 1.83, and contribution rates of 6.59% and 20.87%, respectively.【Conclusion】The study on QTL mapping of vitamin C content would provide a theoretical basis for breeding sweet corn varieties with high quality and nutrition in breeding practice. |
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