| 李德伟，吴建辉，覃振强.低温胁迫对新菠萝灰粉蚧死亡率的影响[J].广东农业科学,2021,48(11):103-111 |
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低温胁迫对新菠萝灰粉蚧死亡率的影响 |
| Effect of Low Temperature Stress on the Mortality of Dysmicoccus neobrevipes Beardsley |
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| DOI：10.16768/j.issn.1004-874X.2021.11.013 |
| 中文关键词: 检疫性有害生物 新菠萝灰粉蚧 剑麻害虫 低温胁迫 致死率 暴露试验 |
| 英文关键词: quarantine pest Dysmicoccus neobrevipes Beardsley sisal pest low temperature stress lethality rate exposure experiment |
| 基金项目:国家自然科学基金（31760540）；广西农业科学院基本科研业务专项（桂农科 2021YT013） |
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| 中文摘要: |
| 【目的】明确低温胁迫对新菠萝灰粉蚧致死效果的影响。【方法】观察新菠萝灰粉蚧不同发育阶段虫态在不同低温（0、4、10 ℃）下 5 个不同处理时间（1、2、4、6、8 h）的致死情况，以常温 26 ℃为对照，分析低温胁迫对该粉蚧死亡率的影响。【结果】0、4、10 ℃低温处理对新菠萝灰粉蚧一龄若虫和二龄若虫死亡率有显著影响，各处理时间的死亡率均大于 30%；二龄若虫经 0、4 ℃低温处理 6、8 h 后死亡率高达 95% 以上；10 ℃低温处理 1、2、4 h 后三龄若虫死亡率均低于 10%，与常温 26 ℃对照差异不显著。未成熟雌成虫在 10 ℃低温处理 4、6 h 后死亡率与常温 26 ℃对照差异不显著。对于成熟雌成虫，10 ℃低温不同处理时间的死亡率均小于 3%，与常温 26 ℃对照差异不显著。【结论】表明低温胁迫对新菠萝灰粉蚧一龄若虫和二龄若虫的死亡率有显著影响，三龄及三龄后若虫在 10 ℃能保持较好的生存能力。 |
| 英文摘要: |
| 【Objective】The effect of low temperature stress on lethality of Dysmicoccus neobrevipes Beardsley（Hemiptera: Pseudococcidae）was determined.【Method】The lethality of D. neobrevipes at different development stages under different low temperatures（0 ℃ , 4 ℃ , 10 ℃）for five different time treatments（1 h, 2 h, 4 h, 6 h, 8 h）was observed in this study, with the compared temperature of 26 ℃ as control. And the effect of low temperature stress on the mortality of D. neobrevipes was analyzed.【Result】There were significant effects on the mortalities of the 1st and 2nd instar nymphs
of D. neobrevipes under low temperature treatments of 0 ℃ , 4 ℃ and 10 ℃ , and the mortality in each treatment was more than 30%. The mortality of the 2nd instar nymph of D. neobrevipes was over 95% after being treated for 6 h and 8 h at the low temperature of 0 ℃ and 4 ℃ . There were no significant differences in the mortality of the 3rd instar nymph of D. neobrevipes after being treated for 1 h, 2 h and 4 h at 10 ℃ compared with that at 26 ℃ , with the mortality of less than 10%. For immature female adults of D. neobrevipes, there were no significant differences in the mortalities after being treated for 4 h, 6 h at 10 ℃ compared with those at 26 ℃ . For mature female adults, there were no significant differences in the mortalities of D. neobrevipes in different treatment times at 10 ℃（less than 3%）compared with those at 26 ℃ .【Conclusion】The results indicated that the low temperature stress had a significant effect on the mortality of the 1st and 2nd instar nymphs of D. neobrevipes, and the development stage of D. neobrevipes after the 3rd instar nymph could maintain a good survival ability at 10 ℃. |
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