| 陈慧琼，彭 程，常晓晓，陈 喆，陆育生，邱继水.黄皮种质资源果实糖组分及其含量特征分析[J].广东农业科学,2022,49(4):8-18 |
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黄皮种质资源果实糖组分及其含量特征分析 |
| Analysis of Soluble Sugar Components and Contents inFruits from Different Wampee Germplasm Resources |
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| DOI：10.16768/j.issn.1004-874X.2022.04.002 |
| 中文关键词: 黄皮 种质资源 糖组分 蔗糖 主成分分析 品质评价 |
| 英文关键词: wampee germplasm resource sugar component sucrose principal component analysis quality evaluation |
| 基金项目:广东省农业科技创新推广及农业资源与生态环境保护建项目（2021KJ269，2021KJ116）；佛山市财政专项资金 -2021 年度共建广东农业科技示范市项目（2021-01）；广东省农业科学院科技人才引进专项（R2021YJYB3022）；国家热带植物种质资源建设项目（NTPGRC2022-009） |
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| 中文摘要: |
| 【目的】分析 64 份黄皮种质资源的果实单果质量、果形指数、可溶性固形物（TSS）含量、
可溶性总糖含量、可滴定酸（TA）含量、糖组分及含量等指标，为选育优质黄皮品种（系）提供依据。
【方法】采用蒽酮比色法和酸碱滴定法测定黄皮果实可溶性总糖和 TA 含量，同时利用高效液相色谱法（HPLC）
分析可溶性糖的组成及含量，结合果形指数、TSS 含量等数据，利用主成分分析方法综合评价筛选优质黄皮品
系。【结果】64 个黄皮品种（系）的单果质量介于 3.82~13.17 g，果形指数 0.94~1.57、平均值为 1.1，TSS 含
量 10.96%~24.55%、平均 18.08%，可溶性总糖含量 8.04%~16.96%、平均 11.88%，TA 含量 0.11%~1.95%、平
均 1.01%，蔗糖含量 18.65~74.85 mg/g、平均 44.28 mg/g，葡萄糖含量 .7~32.1 mg/g、平均 20.00 mg/g，果糖含量
11.15~46.85 mg/g、平均 22.56 mg/g。鹰嘴黄皮（008）可溶性总糖含量最高、达 16.96%，塔下甜皮（086）果糖
占比高达 35.41%、糖酸比达 102.14。【结论】黄皮为蔗糖积累型，通过主成分分析方法筛选综合性状优良品系
10 份，其中早丰黄皮（010）、丛城甜黄皮（050）和金丰黄皮（067）为广东省农作物品种审定委员会已审定品种，
所选品系（种）均可为后续优质黄皮品种选育提供良好的材料基础，也可为黄皮产业品种结构调整提供新选择。 |
| 英文摘要: |
| 【Objective】Fruit quality indexes including single fruit weight, fruit shape index, total soluble solids
(TSS) content, total soluble sugars content, total acids (TA) content and sugar components content of 64 wampee germplasm
resources were investigated in order to provide theoretical basis for the breeding of high-quality wampee cultivars.
【Method】The anthrone colorimetry and acid-base titration method were used to measure the contents of TSS and TA in
wampee fruits, respectively. The sugar component and content were analyzed by High Performance Liquid Chromatography
(HPLC) simultaneously. Taking fruit shape index and TSS into account together, the principal component analysis was conducted to screen out high-quality wampee germplasms. 【Result】The single fruit weight of these cultivars (lines) ranged from
3.82 to 13.17 g, and the fruit shape index was 0.94-1.57, with an average value of 1.1. The TSS ratio was 10.96%-24.55%,
with an average value of 18.08%. The content of total soluble sugar was 8.04%-16.96%, with an average value of 11.88%. The
content of TA ranged from 0.11% to 1.95%, with an average value of 1.01%. The contents of sucrose, glucose and fructose were
18.65-74.85 mg/g, 9.7-32.1 mg/g and 11.15-46.85 mg/g, respectively, while the corresponding average contents were 44.28,
20.00 and 22.56 mg/g. Particularly, the total soluble sugar content of Yingzui wampee (008) was the highest, reaching 16.96%,
while Taxia sweet wampee (086) p ossessed the highest fructose ratio (35.1%) and sugar-acid ratio (102.14).【Conclusion】
Wampee was a type of sucrose accumulation fruit. 10 germplasms with excellent traits were screened out through principal
component analysis comprehensively, of which Zaofeng wampee (010), Congcheng sweet wampee (050) and Jinfeng wampee (067)
had already been released by Guangdong Crop Variety Approval Committee and all the selected germplasms could be used as
candidate materials for breeding of high-quality cultivars and new choice for structure adjustment of wampee varieties. |
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