| 蒋锋,刘鹏飞.甜玉米果皮厚度的遗传分析与QTL定位[J].广东农业科学,2022,(8-9):- |
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甜玉米果皮厚度的遗传分析与QTL定位 |
| nheritance analysis and QTL mapping for pericarp thickness in sweet corn |
| 投稿时间：2022-06-16 修订日期：2022-07-07 |
| DOI： |
| 中文关键词: 甜玉米 果皮厚度 主基因＋多基因 遗传分析 QTL定位 |
| 英文关键词: sweet corn pericarp thickness major genes and polygenes genetic analysis QTL mapping |
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| 中文摘要: |
| 【目的】为研究甜玉米果皮厚度的遗传机理及选育优质的甜玉米品种，对甜玉米果皮厚度性状进行主基因＋多基因遗传分析及QTL定位。【方法】选用果皮厚度性状差异显著的甜玉米自交系T15与T77配制杂交组合。以该组合的F2群体作为试验材料，采用主基因＋多基因混合遗传方法进行遗传模型相关参数分析；结合F2群体各单株的果皮厚度及SSR遗传连锁图谱，利用复合区间作图法对甜玉米果皮厚度进行QTL定位。【结果】甜玉米果皮厚度的最适模型为A-1，即受1对主基因控制的加性和部分显性的遗传模型，主基因遗传率69.10%。在第5、8染色体上分别检测出3个与果皮厚度相关的QTL。其中第5染色体bin5.04区域检测到2个QTL，分别位于标记区间bnlg150-bnlg653和bnlg653-bnlg1208，加性效应值分别为-2.39和-3.01；位于第8染色体的QTL在bin8.03~bin8.04区域，标记区间为umc1741-bnlg2046，加性效应值是-3.06，表型贡献率是22.02%。【结论】甜玉米果皮厚度以主基因效应为主，在育种实践时，可在早期世代进行遗传改良选择。本试验检测到的QTL可用于分子标记辅助选择和品质育种。 |
| 英文摘要: |
| 【Objective】In order to study the genetic mechanism of sweet corn pericarp thickness and select high-quality sweet corn varieties, the major genes and polygenes genetic analysis and QTL mapping of sweet corn pericarp thickness were carried out.【Method】The sweet corn inbred lines T15 and T77 with large differences in pericarp thickness were selected to prepare hybrid combinations. The F2 population of the combination was used as the experimental material, the main genes and polygenes hybrid genetic method was used to analyze the relevant parameters of the genetic model; Combined with the pericarp thickness and SSR genetic linkage map of each individual plant in F2 population, QTL mapping of pericarp thickness in sweet corn was carried out by using composite interval mapping method.【Result】It was found in the experiments that A-1is the optimal model of sweet corn pericarp thickness, that is, an additive and partially dominant genetic model controlled by a pair of major genes and the heritability of major genes was 69.10%. What’s more, there were three QTLs identified on chromosomes 5 and 8 respectively, which were related to pericarp thickness. Two QTLs were detected in region 5.04 of the fifth chromosome 5, which were located in the marker interval bnlg150-bnlg653 and bnlg653-bnlg1208 respectively, and the additive effect values were -2.39 and -3.01 respectively, The QTL located on chromosome 8 was in bin8.03~bin8.04, the marker interval was umc1741-bnlg2046, its additive effect value was -3.06, and the contribution rate to phenotype was 22.02%.【Conclusion】The peel thickness of sweet corn is mainly due to the effect of major genes. In breeding practice, genetic improvement and selection can be carried out in the early generation. The QTLs detected in this experiment can be used for molecular marker assisted selection and quality breeding. |
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