| 麦培婷 1
，梁绍芳 1
，周利长 2
，宫 超 1
，罗少波 1
，孙保娟 1
，衡 周 1
，李 涛 1.不同激素处理对假酸浆种子萌发的影响[J].广东农业科学,2022,49(10):1-8 |
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不同激素处理对假酸浆种子萌发的影响 |
| Effects of Different Hormone Treatments on Germination of Nicandra physaloides Seeds |
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| DOI：10.16768/j.issn.1004-874X.2022.10.001 |
| 中文关键词: 假酸浆 激素浓度 种子萌发 下胚轴 赤霉素 |
| 英文关键词: Nicandra physaloides hormone concentration seed germination hypocotyl gibberellin |
| 基金项目:广东省重点领域研发计划项目（2020B0202090002）；广东省对外科技合作项目（2020A0505020006）；广东省农业科技创新及推广项目（2022KJ106）；广东省农业科学院科技创新战略专项资金（高水平农科院建设）-人才项目（R2020PY-JX003）；广东省农业科学院院长基金（202030） |
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| 中文摘要: |
| 【目的】假酸浆因种子外被胶质严重影响种子的萌发，探究不同处理对假酸浆种子萌发的影响，增加其种子的萌发率。【方法】以假酸浆种子为试材，采用随机区组法，设置种子放置在纱布上 4 ℃低温处理，以及赤霉素（GA3）、6- 苄氨基腺嘌呤（6-BA）和萘乙酸（NAA）的 MS 培养基处理，定期观察并作记录。【结果】与常温对照相比，种子放置在纱布上 4 ℃低温处理显著促进假酸浆种子萌发，以处理 48 h 发芽率最高为 25 个百分点。MS 培养基添加激素处理中，10 mg/L GA3 处理假酸浆种子发芽率和发芽指数分别为 92.00% 和9.58，比 CK 分别提高 4.27 个百分点和 1.33；1 mg/L 6-BA 处理假酸浆种子发芽率最高、为 96.63%，2 mg/L 6-BA处理发芽势最高、为 73.33%，比 CK 分别增加 8.9 个百分点和 10.07 个百分点；1、2 mg/L NAA 处理假酸浆种子发芽率均为 88.86%，但发芽势和发芽指数均比 CK 低；与 CK 相比，10、50 mg/L GA3 处理假酸浆种子下胚轴长度显著增加，而 6-BA 和 NAA 处理的下胚轴长度显著降低。【结论】低温处理能够显著促进假酸浆种子萌发，MS 培养基添加低浓度激素处理均能提高假酸浆种子的发芽率，通过发芽率、发芽势、发芽指数和下胚轴长度综合比较，假酸浆种子的最佳激素处理为 MS+10 mg/L GA3。 |
| 英文摘要: |
| 【Objective】The seed germination of Nicandra physaloides was seriously affected by external colloid.
The study was conducted in order to explore the effects of different hormone treatments on germination of N. physaloides
seeds and increase the seed germination rate.【Method】The seeds of N. physaloides were used as test materials, and the
random block method was used to set 4 ℃ low temperature treatment with seeds put on gauze and MS medium treatments of
gibberellin (GA3
), 6-benzylaminopurine (6-BA) and naphthylacetic acid (NAA), and the results of the four treatments were regularly observed and recorded.【Result】Compared with the normal temperature control, low temperature treatment at 4 ℃
on gauze significantly promoted the germination of N. physaloides seeds, and the highest germination rate was 25% after 48 h of
treatment. The germination rate and germination index of N. physaloides seeds treated with 10 mg/L GA3
were 92.00% and 9.58,
which were 4.27% and 1.33 higher than those of CK, respectively. The germination rate of the seeds treated with 1 mg/L 6-BA
was the highest as 96.63%, and the germination potential of the seeds treated with 2 mg/L 6-BA was the highest as 73.33%,
which were 8.9% and 10.07% higher than those of CK, respectively. The germination rate of the seeds treated with 1 and 2 mg/L
NAA was 88.86%, but both the germination potential and germination index were lower than CK. Compared with CK, 10 and
50 mg/L GA3
treatment significantly increased hypocotyl length, while 6-BA and NAA treatments significantly decreased
hypocotyl length.【Conclusion】Low temperature treatment can significantly promote the germination of N. physaloides seeds,
and low concentration hormone treatment on MS medium can improve the germination rate of N. physaloides seeds. Through
comprehensive comparison of germination rate, germination potential, germination index and hypocotyl length, the best hormone
treatment for N. physaloides seeds is MS + 10 mg/L GA3
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