| 王 蕾 1
，罗帝洲 1
，邓紫璇 1, 2，莫敏莹 1,2，张健玲 3
，苏颖辉 2
，房元杰 1
，张晓爱 1.流式细胞术在农业研究领域中的应用[J].广东农业科学,2022,49(11):66-73 |
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流式细胞术在农业研究领域中的应用 |
| Application of Flow Cytometry in Agricultural Research |
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| DOI：10.16768/j.issn.1004-874X.2022.11.008 |
| 中文关键词: 流式细胞术 农业 畜牧 农作物 农业微生物 |
| 英文关键词: flow cytometry agriculture animal husbandry crop agricultural microorganism |
| 基金项目:广州市科技计划基础与应用基础研究项目（202102080151）；广东省农业科学院农业优势产业学科
团队建设项目（202131TD）；广东省科技专项资金（“大专项 + 任务清单”）项目（江科［2020］182 号，江科［2021］
183 号） |
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| 摘要点击次数: 982 |
| 全文下载次数: 945 |
| 中文摘要: |
| 流式细胞术是一种对悬浮于流体中的微小颗粒进行计数和分选的生物学技术，基于流式细胞仪通
过检测散射或者偶联荧光信号快速、准确、客观、高通量获得悬浮微粒（通常是细胞、细菌等微小颗粒）一系
列重要的生物物理、生物化学相关特征参量，可以根据预选的参量范围对细胞、细菌等微小颗粒进行自动分析
和对特定群体进行分选。流式细胞术在许多科学领域特别是生物技术和医学领域应用广泛，虽然在农业研究中
的应用开始得比较晚，但目前已取得巨大进展。随着流式细胞仪性能的不断提高，标记方法和检测技术的不断
开发与完善，流式细胞术的应用前景将越来越广阔，在农业领域也将发挥越来越重要的作用。介绍了流式细胞
术的工作原理和流式细胞仪功能分类，综述了流式细胞术在农业研究领域中的应用，如对农作物基因组和原生
质体分析以及抗逆研究，对动物免疫、微量元素分析、精子质量和性别控制以及毒素毒性分析，对病原菌和病
毒分析研究等方面的应用和研究进展，展望该研究领域的发展前景，为流式细胞术在农业领域的潜在应用提供
新的思考方向。 |
| 英文摘要: |
| Flow cytometry is a biological technique for counting and sorting out tiny particles suspended in fluid
based on flow cytometer through the detection of scattering or coupled fluorescence signals to obtain a series of important
biophysical and biochemical related characteristics of suspended particles (usually cells, bacteria and other tiny particles)
in a quick, accurate, objective and high-throughput way, and to automatically analyze and sort out specific populations
according to the pre-selected parameter range of cells, bacteria and other tiny particles. Flow cytometry is widely used in many scientific fields, especially biotechnology and medicine. Although the application of flow cytometry in agricultural research
started relatively late, great progress has been made. With the continuous improvement of the performance of flow cytometer,
the continuous development and improvement of labeling methods and detection technologies, the application prospect of
flow cytometry will become wider, and it will also play an increasingly important role in the agricultural field. In this review, it
introduces the working principle and functional classification of flow cytometer, discusses the application and research progress
of flow cytometry in agricultural fields such as crop genome and protoplast analysis, stress resistance research, animal immunity
and trace element analysis, sperm quality and sex control, toxin toxicity analysis, pathogenic bacteria and virus analysis, and
then looks forward to the development prospect of this research field, providing a new direction for the potential application of
flow cytometry in agricultural fields. |
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