| 刘力源 1, 2，李长青 1, 2，姬广磊 3，卢龙飞 1, 2，杨晓斌 2, 4，常丽荣 1, 2.三角褐指藻和小球藻藻粉添加对皱纹盘鲍稚鲍生长的影响[J].广东农业科学,2022,49(12):135-143 |
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三角褐指藻和小球藻藻粉添加对皱纹盘鲍稚鲍生长的影响 |
| Effect of Phaeodactylum tricornutum and Chlorella Powder on the Growth of Juvenile Haliotis discus hannai |
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| DOI：10.16768/j.issn.1004-874X.2022.12.015 |
| 中文关键词: 三角褐指藻 小球藻 皱纹盘鲍 生长 酶活 细菌 |
| 英文关键词: Phaeodactylum tricornutum Chlorella Haliotis discus hannai growth enzyme activity vibrio bacteria |
| 基金项目:国家重点研发计划项目（2018YFD0901400） |
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| 摘要点击次数: 1520 |
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| 中文摘要: |
| 【目的】研究微藻添加对皱纹盘鲍稚鲍生长的影响，探索三角褐指藻和小球藻作为稚鲍饲料添加剂的可行性。【方法】在饲料中添加 1%、3% 和 5% 的三角褐指藻和小球藻藻粉，比较各处理稚鲍的生长参数，全组织总抗氧化能力（T-AOC），超氧化物歧化酶（SOD）、α- 淀粉酶（AMS）、纤维素酶（CL）活性以及内脏囊中细菌、弧菌的数量。【结果】饲料中添加三角褐指藻和小球藻藻粉可显著提高稚鲍壳长，显著降低稚鲍死亡率，其中以 5% 三角褐指藻处理稚鲍的壳长最大（16.38±2.49 mm），1% 小球藻处理稚鲍的死亡率最低（2.00%±0.67%）。饲料中添加三角褐指藻可显著提高稚鲍体质量、增重率，显著降低饵料系数。养殖 40 d 后，5% 三角褐指藻处理和 5% 小球藻处理的 SOD 活性（12.9±0.75、12.99±0.42 U/mg）均显著高于对照。3%、5%三角褐指藻处理的 T-AOC 活性（1.15±0.03、1.19±0.06 U/mg）显著高于对照。5% 三角褐指藻处理的 AMS 活性（4.92±0.74 U/dL）显著高于对照，各处理间的 CL 活性差异不显著。养殖 40 d 后，5% 三角褐指藻和 5% 小球藻处理稚鲍内脏囊中细菌和弧菌的数量均显著低于对照。【结论】饲料中添加三角褐指藻对皱纹盘鲍稚鲍生长的促进效果优于小球藻，以添加 5% 三角褐指藻的效果最优。 |
| 英文摘要: |
| 【Objective】The study aimed to investigate the effect of adding microalgae on the growth of juvenile Haliotis discus hannai, and explore the feasibility of Phaeodactylum tricornutum and Chlorella as feed additives for juvenile abalone.【Method】1%, 3% and 5% of P. tricornutum and Chlorella powder were added to the feed (marked as PT1, PT3, PT5, CH1, CH3, CH5, respectively), and compare the growth parameters, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), α-amylase (AMS) and cellulase (CL) activity of juvenile abalone under various treatments, the number of bacteria and vibrio in the visceral sac were compared.【Result】The addition of P. tricornutum and Chlorella powder to the feed could significantly increase the shell length , and significantly reduce the mortality of juvenile abalone . The shell length of PT5 was the highest (16.38±2.49 mm), and the mortality of CH1 treatment was the lowest (2.007%±0.67%). The addition of P. tricornutum to the feed significantly increased the body weight and weight gain rate, and decreased the feed coefficient. After 40 days of breeding, the SOD activity of PT5 treatment (12.9±0.75 U/mg) and CH5 treatment (12.99±0.42 U/mg) was significantly higher than that of the control group . The T-AOC activity of PT3 and PT5 (1.15±0.03 U/mg,1.19±0.06 U/mg) was significantly higher than that of the control group. The AMS activity of PT5 (4.92±0.74 U/dL) was significantly higher than that of the control group, and there was no significant difference in CL activity among different groups. After 40 days of breeding, the number of bacteria and vibrio in the visceral sac of PT5 and CH5 was lower than that of the control group .【Conclusion】The effect of adding P. tricornutum on the growth of juvenile abalone was better than that of Chlorella, and the effect of adding 5% P. tricornutum was the most significant. |
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