| 姚宗泽 1，杨 艳 2，王世敏 2，刘艳芳 1.基于云南玉米杂交种测试的性状多样性及相关性分析[J].广东农业科学,2023,50(5):30-38 |
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基于云南玉米杂交种测试的性状多样性及相关性分析 |
| Diversity and Correlation Analysis of Traits based on Yunnan Maize Hybrids Testing |
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| DOI：10.16768/j.issn.1004-874X.2023.05.004 |
| 中文关键词: 杂交玉米 DUS 测试性状 多样性 相关性 相似系数 |
| 英文关键词: hybrid maizes DUS testing traits diversity correlation similarity coefficient |
| 基金项目:农业农村部科技发展中心委托测试品种 DUS 测试项目（DUSWT11） |
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| 中文摘要: |
| 【目的】评价当前玉米品种特异性（Distinctness）、一致性（Uniformity）、稳定性（Stability）测试（DUS 测试）性状在玉米杂交品种上的应用。【方法】以 2020—2021 年进行玉米 DUS 测试的云南地区 89 个杂交品种为材料，在测试数据基础上，对 38 个测试性状进行多样性、相关性分析，以及对 89 个品种进行聚类分析。【结果】38 个性状在 89 个玉米杂交品种中共检测到 191 个表达状态，平均每个性状检测到 5.0263 个，变幅为 2~8 个；平均有效表达状态为 2. 8217，变幅为 1.0227~6.0697；38 个性状平均 Shannon-Weaver 多样性指数为 1.0669，变幅为 0.0616~1.9069。不同性状的多样性表现差异较大，有的性状在 89 个玉米品种中仅出现 2 种表达状态，有效表达状态数和 Shannon-Weaver 多样性指数均比较低；有些性状在 89 个玉米品种中出现 8 种表达状态，有效表达状态数和 Shannon-Weaver 多样性指数均比较高。38 个测试性状中，有 12 对性状间存在极显著正相关关系，散粉期与抽丝期、植株穗位高度与穗位高 / 株高比率间的相关性系数较高，分别为 0.94 和 0.90。上述结果表明，当前玉米 DUS 测试指南中测试性状在杂交种中的多样性较好，品种区分力强，对存在极显著相关的性状，在特异性判定、实质性派生品种判定时需作特殊考虑，可以根据其重要性赋予不同权重，以校准判定结果。聚类分析显示，89 个杂交种的相似系数为 0.24~0.53，可聚类为 5 大类群，其中第Ⅳ类群包含 81 个品种（占 91.0%），该类群中最相近的品种相似性系数为 0.53，表明云南玉米杂交种多样性较好、品种间差异明显。【结论】当前玉米 DUS 测试性状在玉米杂交品种测试依然保持较高的区分力，测试性状多样性丰富，且有 12 对测试性状间存在极显著的正相关性。 |
| 英文摘要: |
| 【Objective】To evaluate the application of distinctness, uniformity, and stability (DUS) testing traits in maize hybrids.【Method】89 hybrid maize varieties in Yunnan Province for DUS testing in 2020-2021 were used as materials, based on the tested data, diversity and correlation analysis were conducted for 38 testing traits, and cluster analysis was conducted for 89 hybrids.【Result】A total of 191 expression states were detected for 38 traits in 89 maize hybrids, with an average of 5.0263 detected for each trait, with a range of 2~8. The average effective expression state was 2.8217, with a range of 1.0227~6.0697. The average Shannon-Weaver diversity index for 38 traits was 1.0669, with a range of 0.0616～1. 9069. The diversity performance of different traits is varies greatly, with some traits showing only 2 expression states in 89 maize varieties, and the number of effective expression states and Shannon-Weaver diversity index are relatively low. There are 8 expression states for some traits in 89 maize varieties, and the number of effective expression states and Shannon-Weaver diversity index are relatively high. Among the 38 testing traits, 12 pairs of traits had a highly significant positive correlation. The correlation coefficients between pollination stage and heading stage, and between plant spike height and plant spike height/plant height ratio were higher, 0.94 and 0.90, respectively. The above results indicate that the diversity of the tested traits in the current maize DUS testing guidelines in hybrids is good, and the variety differentiation is strong. It is also suggested that there are traits with significant correlations among traits, and special considerations should be taken in the determination of specificity and substantial derived varieties, different weights can be given based on their importance to calibrate the judgment results. Cluster analysis showed that the similarity coefficient of 89 hybrids ranged from 0.24 to 0.53, which could be clustered into five major groups. Group IV included 81 varieties, accounting for 91.0%, the similarity coefficient of the closest variety in this group was 0.53, indicating that Yunnan maize hybrids had good diversity and significant differences among varieties.【Conclusion】The current maize DUS testing traits still maintain high discrimination in maize hybrid varieties testing, with rich diversity of testing traits and a highly significant positive correlation between 12 pairs of testing traits. |
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