| 付丹文 1,2，陈亚慧 1
，杨少华 1
，高 峰 1.紫心甘薯 IbMYB1 上游 7 个调控蛋白的互作与表达分析[J].广东农业科学,2023,50(6):1-9 |
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紫心甘薯 IbMYB1 上游 7 个调控蛋白的互作与表达分析 |
| Interaction and Expression Analysis of 7 Transcription Proteins Upstream of IbMYB1 in Purple-Fleshed Sweet Potato |
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| DOI：10.16768/j.issn.1004-874X.2023.06.001 |
| 中文关键词: 紫心甘薯 花色素苷生物合成 转录因子 IbMYB1 上游调控蛋白互作 表达分析 |
| 英文关键词: purple-fleshed sweet potato anthocyanin biosynthesis transcription factor IbMYB1 transcription proteins interaction expression analysis |
| 基金项目:广东省基础与应用基础研究基金（2022A1515110300） |
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| 中文摘要: |
| 【目的】对前期筛选到的紫心甘薯 IbMYB1 上游 7 个调控蛋白（IbERF1、IbPDC、IbPGP19、
IbSCF、IbWRKY1、IbJOX4 和 IbEIN3-2）的互作与表达进行分析，进一步阐明花色素苷在特定时空合成与积累
的调控网络。【方法】采用酵母双杂交和双分子荧光互补试验，对 7 个调控因子之间的相互作用进行检测，使
用荧光定量 PCR 对紫心甘薯和白心甘薯根不同发育时期花色素苷合成调控和筛选的上游调控因子进行基因表达
特征分析。【结果】IbERF1 与 IbSCF、IbWRKY1 存在相互作用。双分子荧光互补试验证实了酵母双杂交的试验
结果。IbERF1 基因在紫心甘薯根不同时期的表达量均低于白心甘薯，且随着根中花色素苷的积累其表达量逐渐
降低，IbWRKY1 和 IbSCF 基因在紫心甘薯和白心甘薯根不同发育时期中的表达量，与花色素苷的积累无明显的
相关关系。【结论】IbMYB1 上游调控蛋白 IbERF1、IbSCF 可分别与 IbWRKY1 形成复合物，以转录复合体的形
式共同调控 IbMYB1 表达。推测 IbERF1 可能负调控花色素苷的合成，IbSCF 和 IbWRKY1 参与紫心甘薯花色素
苷的合成与调控，可能还存在更为复杂的机制。 |
| 英文摘要: |
| 【Objective】The interaction and expression of 7 upstream transcription proteins ( IbERF1, IbPDC, IbPGP19,
IbSCF, IbWRKY1, IbJOX4 and IbEIN3-2 ) of purple-fleshed sweet potato IbMYB1 were analyzed to further elucidate the
regulatory network of anthocyanin synthesis and accumulation in specific time and space.【Method】Yeast two-hybrid and
BiFC experiment were used to detect the interactions between the 7 upstream transcription regulators. RT-qPCR was used
to analyze the gene expression traits of an thocyanin synthesis regulation and upstream regulators in purple-fleshed sweet
potato and white-fleshed sweet potato at roots different developmental stages.【Result】IbERF1 interacted with IbSCF and
IbWRKY1. BiFC experiment confirmed the results of yeast two-hybrid experiment. The expression level of IbERF1 gene in
purple-fleshed sweet potato roots was lower than that in white-fleshed sweet potato at different periods, and the expression
level decreased gradually with the accumulation of anthocyanin in root. The expression levels of IbWRKY1 and IbSCF genes in
the roots of purple-fleshed sweet potato and white-fleshed sweet potato at different developmental stages showed no significant
positive or negative correlation with anthocyanin accumulation.【Conclusion】The upstream regulatory proteins IbERF1 can
form a complex with IbSCF and IbWRKY1, respectively to co-regulate the expression of IbMYB1 in the form of a transcription
complex. It is speculated that IbERF1 may negatively regulate anthocyanin synthesis, and IbSCF and IbWRKY1 may be
involved in the synthesis and regulation of anthocyanin in purple-fleshed sweet potato. |
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