宁都黄鸡 GH 基因 SNP 与鸡冠性状的关联分析

马荆鄂; 曾成瑶; 周 敏; 谭玉文; 朱学农; 饶友生; 许继国

文章摘要

马荆鄂，曾成瑶，周敏，谭玉文，朱学农，饶友生，许继国.宁都黄鸡 GH 基因 SNP 与鸡冠性状的关联分析[J].广东农业科学,2023,50(7):137-145

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Association Between SNP of GH Gene and Comb Traits of Ningdu Yellow Chicken

DOI：10.16768/j.issn.1004-874X.2023.07.014

中文关键词: 宁都黄鸡生长激素基因单核苷酸多态位点（SNP）鸡冠冠高冠长

英文关键词: Ningdu yellow chicken growth hormone gene single nucleotide polymorphism (SNP) comb comb height comb length

基金项目:国家自然科学基金（32160784）；江西省教育厅科技计划项目（ GJJ2202001，GJJ2202002）；江西省重点研发计划重点项目（20212BBF61011）

作者	单位
马荆鄂，曾成瑶，周敏，谭玉文，朱学农，饶友生，许继国	南昌师范学院生物技术研究院 / 江西省地方鸡种遗传改良重点实验室，江西南昌 330032

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中文摘要:

【目的】研究宁都黄鸡 GH 基因（Growth hormone，GH）5'- 侧翼和内含子 1 区域单核苷酸多态性（Single nucleotide polymorphism，SNP）与鸡冠性状的关联性，以期筛选地方鸡种鸡冠性状的早期选育分子标记。【方法】以 499 只宁都黄鸡公鸡为研究对象，测定 7 个不同周龄的冠高、冠长、冠厚、肉垂长、肉垂厚以及冠齿数指标，利用 SAS（Statistical analysis system）分析 GH 基因部分序列 SNP 与鸡冠性状的关联性。【结果】有 13 个 SNP 与冠高性状指标显著关联（P < 0.05），即 C1272225T、G1272611A、C1271211T、C1270902T、A1270769G、G1270640C、T1270578C、G1270503C、T1270451C、C1270032T，其中 A1270769G 与 7 个周龄冠高指标均显著关联；G1272611A 与 5 个周龄冠高指标显著关联；有 10 个 SNP 与冠长性状显著关联（P < 0.05），即 C1272225T、G1272611A、C1271211T、C1270902T、A1270769G、G1270640C、T1270578C、G1270503C、T1270451C、C1270032T，其中 C1271211T 与 6 个周龄冠长指标显著关联，G1272611A 与 5 个周龄冠长指标显著关联。G1272611A 位点与 5 个连续周龄冠长或冠高性状指标均显著关联，该位点在该群体中对应 GG 和 GA 2 种基因型，GG 基因型个体的 5 个连续周龄冠高或冠长均显著高于 GA 基因型个体。【结论】G1272611A 位点可作为宁都黄鸡冠高和冠长性状选育重要候选分子标记。

英文摘要:

【Objective】The Paper aims to explor the relationship between single nucleotide polymorphisms (SNP) of 5'-flanking and Intron 1 in growth hormone (GH) gene and the comb traits of Ningdu yellow chicken, and find molecular markers for the comb traits selection of local chicken breeds. 【Method】The comb trait data at seven different week age of 499 Ningdu yellow chickens were recorded including comb height, comb length, comb thickness, wattle length, wattle thickness and number of comb teeth. These traits were associated with SNPs in the 5'- flanking and Intron 1 regions of GH gene using statistical analysis system (SAS). 【Result】A total of 13 SNPs were significantly associated with comb height trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. A1270769G was significantly correlated with the comb height traits at seven different week age. G1272611A was significantly associated with the comb height traits at five different week age. A total of 10 SNPs were significantly associated with comb length trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. C1271211T was significantly correlated with the comb length at six different week age. G1272611A was significantly correlated with the comb length at five different week age. The locus G1272611A was significantly associated with both comb length and height trait at five consecutive weeks, which had two genotypes in this population, GG and GA, comb height or length of GG genotype individuals were significantly higher than those of GA genotype. 【Conclusion】G1272611A could be used as a candidate molecular marker for selection of Ningdu yellow chicken comb traits.

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