| 桑贤东,杨晓慧,徐 斌,等.基于靶向代谢组学的大红花不同花色中花青素差异分析[J].广东农业科学,2024,(8-10):- |
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基于靶向代谢组学的大红花不同花色中花青素差异分析 |
| Analysis of Anthocyanin in Different Flower colors of Hibiscus rosa-sinensis Based on Targeted Metabolomics |
| 投稿时间：2024-05-28 修订日期：2024-06-23 |
| DOI： |
| 中文关键词: 大红花 花色 代谢组学 花青素 合成通路 超高效液相色谱–串联质谱 |
| 英文关键词: Hibiscus rosa-sinensis flower color metabolomics anthocyanins synthetic pathway ultra performance liquid chromatography-tandemmass spectrometry(UPLC-MS/MS) |
| 基金项目:汕尾市省科技专项资金项目(220130105850566)；广东省自然科学基金（2214050006239） |
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| 摘要点击次数: 15 |
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| 中文摘要: |
| 【目的】大红花（Hibiscus rosa-sinensis L.）因花色丰富、观赏价值较高，被广泛应用于华南地区的城市绿化与园林造景中。研究大红花不同花色中花青素种类及含量的特征差异，分析大红花品种间代谢物特异性及共同性特征，为深入挖掘品种特性及花色定向育种提供理论和技术支撑。【方法】利用靶向代谢组学技术对白色（W）、红色（R）、黄色（Y）3种大红花不同花色花瓣组织中的花青素苷组成和含量进行测定，利用Kyoto?Encyclopedia?of?Genes?and?Genomes（KEGG）数据库、生物学软件及多元统计学方法对代谢物进行功能注释、鉴定与分析。【结果】大红花W、R与Y花色中共检测出90种代谢物，其中差异代谢物共有78种，主要为矢车菊素（41.11%）、飞燕草素（20.00%）与芍药花素（13.33%）等。不同花色间差异代谢物分别为62个（Y vs R）、50个（Y vs W）和65个（R vs W），其中共有差异代谢物22个。综合考虑各个比较组合差异显著性和绝对含量，发现矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷在红色花中含量较高；飞燕草素-3-O-(6'-O-酒石酰)葡萄糖苷、原花青素B4和原花青素B3在黄色花含量较高。KEGG代谢通路分析发现差异代谢物在不同花色的组织中显著富集。【结论】矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷是大红花呈红色的重要代谢物质，原花青素B3是花色呈黄色的重要花青素类代谢物。该结果初步揭示了大红花不同花色的代谢物差异，可为大红花花色形成机制和花色改良提供理论参考。 |
| 英文摘要: |
| 【Objetive】]Hibiscus rosa-sinensis L has high ornamental value due to rich flower color and is widely used in urban greening and landscape architecture in South China. In order to further provide theoretical and technical support for in-depth excavation of variety characteristics and flower color directional breeding technology, the anthocyanin composition, content, and the specificity and common anthocyanins of different flower color for H. rosa-sinensis L were detected and analyzed in this study. 【Method】The anthocyanin composition and content of three different Petal tissues of white (W), red (R) and yellow (Y) flowers were determined used targeted metabolomics. Functional annotation, identification, and analysis of metabolites were performed using the Kyoto ?Encyclopedia ?of ?Genes ?and ?Genomes (KEGG) databases, biological software and multivariate statistical methods.【Result】The results showed that a total of 90 metabolites were detected in W, R and Y colors for H. rosa-sinensis L, among which 78 were differential metabolites, including Cyanidin (41.11%), Delphinidin (20.00%) and Peonidin (13.33%), etc. There were 62 (Y vs R), 50 (Y vs W), and 65 (R vs W) differential metabolites among different flower colors, with a total of 22 differential metabolites. Comprehensively considering the significance and absolute contents of differences in different comparative combinations, the cyanidin-3-O-sambubioside-glucoside, cyanidin-3-gentiobioside, pelargonidin-3-O-sophoroside, and peonidin-3-O-glucoside were relatively high in red flower; The Delphinidin-3-O-(6'-O-tartaryl) glucoside, procyanidin B3, procyanidin B4 were relatively high in yellow flower. KEGG metabolic pathways analysis revealed that differential metabolites were significantly enriched in different flower color tissues. 【Conclusion】 The cyanidin-3-O-sambubioside-glucoside, cyanidin-3-gentiobioside, pelargonidin-3-O-sophoroside, and peonidin-3-O-glucoside were important metabolites determining the flower red color, while procyanidin B3 was an important metabolite determining the flower yellow color. This result preliminary revealed the differences in metabolites among different colors of saffron, which could provide theoretical references for mechanism of color formation and color improvement for H. rosa-sinensis L. |
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