| 乐翔兆.草地贪夜蛾bHLH基因家族鉴定与生物信息学分析[J].广东农业科学,2024,(8-9):- |
|
PDF
HTML
草地贪夜蛾bHLH基因家族鉴定与生物信息学分析 |
| Genome-wide Identification and Bioinformatics Analysis of bHLH Gene Family in Spodoptera frugiperda |
| 投稿时间：2024-06-02 修订日期：2024-07-17 |
| DOI： |
| 中文关键词: 草地贪夜蛾 碱性螺旋-环-螺旋 转录因子 基因家族 功能结构 |
| 英文关键词: Spodoptera frugiperda bHLH transcription factor gene family functional structure |
| 基金项目:江西省教育厅科学技术研究项目 |
|
| 摘要点击次数: 14 |
| 全文下载次数: 0 |
| 中文摘要: |
| 【目的】草地贪夜蛾（Spodoptera frugiperda J. E. Smith）是对我国粮食安全构成巨大威胁的重要入侵害虫，碱性螺旋-环-螺旋(basic helix-loop-helix, bHLH)基因家族转录因子在昆虫生长发育过程发挥重要调控作用，因此可能成为RNAi农药的备选靶标基因。为确定草地贪夜蛾bHLH基因相关靶标，鉴定分析了草地贪夜蛾bHLH基因家族成员信息。【方法】基于全基因组数据，对草地贪夜蛾bHLH基因家族成员的理化性质、亚细胞定位、蛋白保守结构域、系统发育进化、基因染色体定位、蛋白质三级结构预测等内容进行分析。【结果】在草地贪夜蛾基因组中鉴定到56个bHLH基因，编码54条不同的蛋白序列。草地贪夜蛾bHLH转录因子氨基酸数目为84～2557，等电点为4.88～10.76，相对分子质量为9,751.19～285,517.20。草地贪夜蛾bHLH基因家族成员分为A至F六组，分别有25、9、10、1、10、1个基因，分别编码24、9、10、1、9、1条不同的蛋白序列。同组成员有相似类型的motif，且同一分组的bHLH结构域在系统发育进化分析中大部分归于同一分枝，表明其在进化中具有保守性。草地贪夜蛾A组成员HLH4C（Sfru006728.1）及B组成员鸟苷酸环化酶Gyc76C（Sfru012226.1）被预测包含bHLH的同时，还包含鸟苷酸代谢相关基因的保守结构域，与同组其他基因相比也具有更复杂的三级结构，推测两者可能源自草地贪夜蛾相关的鸟苷酸代谢基因与bHLH基因在进化上发生复制与重叠，并可能具有未知的复杂的生理功能。【结论】鉴定分析获得的预测的草地贪夜蛾bHLH基因家族成员，获得其序列及理化、亚细胞定位、结构及进化等多方面信息，有助于进一步研究草地贪夜蛾bHLH转录因子家族成员功能，以及筛选草地贪夜蛾RNAi农药靶标。 |
| 英文摘要: |
| 【Objective】Spodoptera frugiperda J. E. Smith is an important invasive pest that poses a great threat to food security in China. Basic helix-loop-helix (bHLH) gene family transcription factors play important roles in insect growth and development. Therefore, they may be candidate target genes for RNAi pesticides. This study explores alternative targets for such pesticides by investigating the bHLH gene family in S. frugiperda.【Methods】Based on the whole genome data, the physicochemical properties, subcellular localization, conserved protein domain, phylogenetic evolution, gene chromosome localization, and protein tertiary structure prediction of the bHLH gene family members in S. frugiperda were analyzed.【Results】56 bHLH genes were identified in the genome of S. frugiperda, encoding 54 different protein sequences. The amino acid number of SfbHLH transcription factors was 84—2557, the isoelectric point was 4.88—10.76, and the relative molecular weight was 9,751.19—285,517.20. The members of the bHLH gene family were divided into six groups from A to F, with 25, 9, 10, 1, 10 and 1 genes encoding 24, 9, 10, 10 and 1 different protein sequences, respectively. Members of the same group have similar types of motifs, and most bHLH domains of the same group are grouped together within the same branch in phylogenetic analysis, indicating that they are evolutionarily conserved. Group A member HLH4C (Sfru006728.1) and Group B member guanylate cyclase Gyc76C (Sfru012226.1) were predicted to contain not only bHLH, but also the conserved domain of genes related to guanylate metabolism, and they have more complex tertiary structure than other genes in the same group. It is speculated that both of them may be derived from the duplication and overlap of guanylate metabolism genes and bHLH genes in evolution, and may have unknown complex physiological functions.【Conclusion】The predicted bHLH gene family members were identified and analyzed to obtain their sequence, physicochemical, subcellular location, structure and evolution information, which is helpful to further study the functions of the bHLH transcription factor family members and screen the RNAi pesticide targets of S. frugiperda. |
|
查看/发表评论 下载PDF阅读器 |
|
|
|
