文章摘要
张彬,林原有,原宝龙,等.氮、磷、碳和铁源及浓度对波吉卵囊藻生长性能的影响[J].广东农业科学,2024,(9-11):-
PDF    HTML 氮、磷、碳和铁源及浓度对波吉卵囊藻生长性能的影响
ZHANG Bin1,LIN Yuan-you1, YUAN Bao-long1,2, ZHU Xi-en1, CHEN Tian-cong1, ZHAO Yong-zhen1, SHEN Nai-kun2*
投稿时间:2024-06-09  修订日期:2024-07-11
DOI:
中文关键词: 波吉卵囊藻  氮源  磷源  碳源  铁源  藻细胞密度
英文关键词: Oocystis Borgei  Nitrogen source  Phosphorus source  Carbon source  Iron source  Algal cell density
基金项目:广西科技重大专项“南美白对虾设施化生态养殖模式研发与示范”(桂科AA23062047);“现代农业产业技术体系国家虾蟹产业技术体系防城港对虾综合试验站”(CARS-48-53);广西水产科学研究院结余经费科技项目“基于生物饵料和水质生物调控的凡纳滨对虾生态育苗技术研究”(GXIFJY-202310)。
作者单位邮编
张彬 广西水产科学研究院 530021
林原有 广西水产科学研究院 
原宝龙 广西水产科学研究院广西民族大学海洋与生物技术学院 
朱昔恩 广西水产科学研究院 
陈田聪 广西水产科学研究院 
赵永贞 广西水产科学研究院 
申乃坤* 广西民族大学海洋与生物技术学院 530006
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中文摘要:
      【目的】研究氮、磷、碳和铁营养盐对波吉卵囊藻(Oocystis borgei)生长的影响,筛选出最佳营养盐配方,为其高效扩培提供参考。【方法】先利用单因素试验分析不同氮源、磷源、碳源和铁源对波吉卵囊藻生长的影响,获得各自最佳营养盐及最适浓度,再利用正交试验对波吉卵囊藻生长影响较大因素进行优化,得出最佳营养盐的配方。【结果】单因素试验结果表明,波吉卵囊藻的最佳氮、磷、碳和铁源及浓度分别是:CO(NH2)2 160 mg/L、C3H7NaO6P 8 mg/L、NaHCO3 2 g/L、FeCl3 0.2 mg/L;正交试验结果表明,各营养盐对波吉卵囊藻生长影响大小为:磷源>氮源>碳源,其中磷源对波吉卵囊藻生长影响最为显著(P<0.05),各营养盐的最适浓度为:CO(NH2)2 120 mg/L、C3H7Na2O6P 12 mg/L、NaHCO3 2.5 g/L。最优条件下藻细胞密度可达22.07×106 cell/mL,比优化前(1.74×106 cell/mL)提高12倍以上。【结论】本研究获得适合波吉卵囊藻培养的最佳配方,为下一步大规模培养提供了理论参考。
英文摘要:
      【Objective】The optimal conditions of nitrogen, phosphorus, carbon and iron nutrient concentration affecting the growth of Oocystis borgei were explored, and the optimal nutrient formula was selected to provide data for its large-scale and efficient expansion.【Method】Single factor test was used to analyze the effects of different nitrogen, phosphorus, carbon and iron nutrients on the growth of O. borgei, and to obtain the best nutrients and their respective concentrations. Orthogonal tests were conducted on the best nitrogen, phosphorus and carbon sources and concentrations. Finally, the optimal nutrient formula was obtained.【Result】The results of single factor experiment showed that the optimal sources and concentrations of nitrogen, phosphorus, carbon and iron for O. borgei were CO(NH2)2 160 mg/L, C3H7NaO6P 8 mg/L, NaHCO3 2 g/L and FeCl3 0.2 mg/L, respectively. The results of orthogonal test showed that the effect of each nutrient on the growth of O. borgei was in the order of phosphorus source > nitrogen source > carbon source, and phosphorus source had the most significant effect on the growth of O. borgei (P<0.05). The optimal concentration of each nutrient was: CO(NH2)2 120 mg/L, C3H7NaO6P 12 mg/L, NaHCO3 2.5 g/L. Under the optimal condition, the algal density reached 22.07×106 cells /mL, which was 12 times higher than that before optimization (1.74×106 cells /mL). 【Conclusion】This study obtained the best formula for the cultivation of O. borgei, which provides theoretical reference for the next large-scale cultivation.
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