| 张彬,林原有,原宝龙,等.营养盐对波吉卵囊藻生长性能的影响[J].广东农业科学,2024,(10):- |
| Effects of nutrient salts on growth performance of Oocystis porgii |
| 投稿时间：2024-06-09 修订日期：2024-11-21 |
| DOI： |
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| 摘要点击次数: 267 |
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| Abstract: |
| 【Objective】The optimal conditions of nitrogen, phosphorus, carbon and iron nutrient concentrations affecting the growth of Oocystis borgei were explored, and the optimal nutrient formula was selected to provide data for its large-scale and efficient expansion.【Method】Single factor test was used to analyze the effects of different nitrogen, phosphorus, carbon and iron nutrients on the growth of O. borgei, and to obtain the best nutrients and their respective concentrations. Orthogonal tests were conducted on the best nitrogen, phosphorus and carbon sources and concentrations. Finally, the optimal nutrient formula was obtained.【Result】The results of the single factor experiment showed that the optimal sources and concentrations of nitrogen, phosphorus, carbon and iron for O. borgei were CO(NH2)2 160 mg/L, C3H7NaO6P 8 mg/L, NaHCO3 2 g/L and FeCl3 0.2 mg/L, respectively. The results of the orthogonal test showed that the effect of each nutrient on the growth of O. borgei was in the order of phosphorus source > nitrogen source > carbon source, and phosphorus source had the most significant effect on the growth of O. borgei (P<0.05). The optimal concentration of each nutrient was: CO(NH2)2 120 mg/L, C3H7NaO6P 12 mg/L and NaHCO3 2.5 g/L. Under the optimal condition, the algal density reached 22.07×106 cells/mL, which was 12 times higher than that before optimization (1.74×106 cells/mL). 【Conclusion】This study determined the optimal nitrogen, phosphorus, carbon, and iron sources and concentrations for Bogia ovata and optimized the nutrient salt formula to significantly increase algal cell density. |
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