| 康奎,唐艳龙,龚俊,等.入侵贵州贵阳草地贪夜蛾的分子鉴定及适应性研究[J].广东农业科学,2024,(8-9):- |
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入侵贵州贵阳草地贪夜蛾的分子鉴定及适应性研究 |
| Molecular identification and adaptation study of invasive Spodoptera frugiperda in Guiyang, Guizhou province |
| 投稿时间：2024-06-13 修订日期：2024-07-18 |
| DOI： |
| 中文关键词: 草地贪夜蛾，水稻型，玉米型，分子标记，寄主植物，适应性 |
| 英文关键词: Spodoptera frugiperda rice strain corn strain molecular marker host plants |
| 基金项目:贵州省教育厅创新群体项目(黔教合KY字[2020]026)；遵义市科技局重点项目(遵市科合 HZ 字(2021)195 号)；国家自然科学基金青年科学(32202286) |
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| 摘要点击次数: 13 |
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| 中文摘要: |
| 【目的】贵州省为草地贪夜蛾的周年繁殖区，采用分子标记来鉴定贵州贵阳草地贪夜蛾的种群生物类型，并对其在玉米、高粱、水稻上的适应性进行研究，有利于明确入侵贵州贵阳的草地贪夜蛾的种群生物型并了解其生物型特性。【方法】采用CO Ⅰ和Tpi分子标记方法，对贵州贵阳3个县市共计32个草地贪夜蛾样本进行扩增、测序和比对分析，明确入侵贵州贵阳的种群的生物型，并记录草地贪夜蛾各龄期幼虫取食3种寄主植物后的发育历期、成虫羽化率和存活时长，评估草地贪夜蛾对3种寄主植物的适应性。【结果】基于线粒体CO Ⅰ基因分析结果显示，32个草地贪夜蛾样本中，93.75%为水稻型，6.25%为玉米型比例，且其序列特征与相应生物型的草地贪夜蛾种群具有很高的一致性，而基于核基因组Tpi基因分析结果表明所有样品单倍型特点均表现为玉米型。适应性研究结果显示入侵贵阳的草地贪夜蛾在玉米上的幼虫期最短（13.22 d）、成虫存活时间最长（16.42 d），且羽化率最高（95.14 %），在高粱上次之，在水稻上的幼虫期最长（16.59 d）、成虫存活时间最短（8.56 d），羽化率也最低（75.15 %）。【结论】研究结果有助于分析入侵贵州省的草地贪夜蛾来源，同时为高粱的草地贪夜蛾防控提供了一定的基础，对当地有关部门快速进行精准监测及防控有着重要意义。 |
| 英文摘要: |
| 【Objective】As Guizhou Province serves as an annual breeding area for S. frugiperda, the use of molecular markers to identify the biotype of S. frugiperda population in Guiyang, Guizhou, and studying its adaptability to corn, sorghum, and rice is crucial for clarifying the biotype of S. frugiperda invading Guiyang and understanding its biotype characteristics. 【Method】 Utilizing CO I and Tpi molecular marker methods, 32 fall armyworm samples from three counties and cities in Guiyang, Guizhou, were amplified, sequenced, and analyzed for comparison. This was done to determine the biotype of the invading population in Guiyang. The developmental duration, adult emergence rate, and survival duration of fall armyworm larvae of different instars after feeding on the three host plants were recorded to evaluate the adaptability of the fall armyworm to these host plants. 【Result】Based on mitochondrial CO I gene analysis, the results showed that 93.75% belonged to the rice biotype, while the corn biotype accounted for 6.25%. The sequence characteristics were highly consistent with the corresponding biotypes of S. frugiperda population. Meanwhile, the analysis of nuclear genome Tpi gene revealed that all samples exhibited the haplotype characteristics of the corn biotype. The adaptability study indicated that the invading S. frugiperda in Guiyang had the shortest larval period, longest adult survival time, and highest emergence rate on corn, followed by sorghum. In contrast, it had the longest larval period, shortest adult survival time, and lowest emergence rate on rice. 【Conclusion】The results of this study are beneficial for analyzing the origin of S. frugiperda invading Guizhou Province. It also provides a basis for the prevention and control of S. frugiperda on sorghum, which is of great significance for local authorities to conduct rapid and precise monitoring and prevention measures. |
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