| 何雪雯,赵浩航,温依铭,等.大口黑鲈源维氏气单胞菌致病菌的分离鉴定 及其毒力和耐药基因分析[J].广东农业科学,2024,(7):- |
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大口黑鲈源维氏气单胞菌致病菌的分离鉴定 及其毒力和耐药基因分析 |
| Isolation, Identification, virulence and drug resistance genes analysis of Aeromonas veronii from Micropterus salmoides |
| 投稿时间：2024-06-17 修订日期：2024-07-31 |
| DOI： |
| 中文关键词: 加州鲈 维氏气单胞菌 毒力基因 耐药基因 |
| 英文关键词: Micropterus salmoides Aeromonas veronii virulence resistance genes drug resistance genes |
| 基金项目:国家重点研发计划项目（2020YFD0900201）；中山市社会公益与基础研究项目(2021B2056) |
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| 中文摘要: |
| 摘 要：【目的】分离加州鲈（Micropterus salmoides）患病组织中的致病菌，鉴定致病菌的生理特性，分析其毒力、耐药基因，以期指导临床科学用药。【方法和结果】从广东湛江某养殖鱼厂的患病加州鲈（M. salmoides）病变组织中分离出菌落形态呈圆形、表面光滑呈乳白色、不透明的细菌，编号为MSB-2。经革兰氏染色、生理生化反应等表型鉴定革兰氏阴性菌——气单胞菌（Aeromonas）。通过扩增 16S rRNA基因序列并构建系统发育树，进一步鉴定该菌为维氏气单胞菌（Aeromonas veronii）。该菌株具有act、aer、aexT等毒力相关基因及tetC、atetA、Sull等耐药相关基因。药敏试验结果表明，该菌对羧苄西林、多西环素、头孢氨苄、新霉素、庆大霉素、头孢拉定、米诺环素、头孢唑林等药物敏感。对健康加州鲈进行人工回归感染病原菌后，出现与病鱼相似的临床症状，如腹鳍及胸鳍基部充血、肛门红肿、腹部明显膨大等，解剖发现腹腔有大量积液，与自然患病的症状相同。【结论】维氏气单胞菌是引起此次加州鲈疾病的致病菌，养殖过程中可选用新霉素、多西环素等批准渔用药物进行防治。 |
| 英文摘要: |
| Abstract: 【Objective】The causative agent was isolated from the diseased fish (Micropterus salmoides) tissue. In this experiment, we characterized the physiological properties of the pathogenic bacteria, analyzed their virulence genes and drug resistance genes, and hoped to use these data to guide the scientific use of drugs in clinical practice.【Method】Samples were collected from diseased tissue of diseased fish from a fish factory in Zhanjiang, Guangdong Province, China. The bacteria were isolated by dilution coating method, and the 16S rRNA sequence comparison and biochemical tests were performed to identify the species, and the virulence genes, drug resistance genes and drug sensitivity characteristics were also identified.【Result】Bacteria with rounded colony morphology, smooth milky white surface and opaque color were isolated from diseased tissues of M. salmoides, and numbered as MSB-2. Phenotypic identification of Gram-negative bacteria by Gram staining and physiological and biochemical reactions——Aeromonas. By amplifying the 16S rRNA gene sequence and constructing a phylogenetic tree, the bacterium was further identified as Aeromonas veronii. The strain has virulence-related genes such as act, aer and aexT and resistance-related genes such as tetC, atetA and Sull. The results of the drug sensitivity test showed that the bacterium was sensitive to Carbenicillin, Doxycycline, Cefalexin, Neomycin, Gentamicin, Cefradine, Minocycline, Cefazolin and so on. Artificial regression of healthy fish infected with A. veronii showing clinical signs similar to those of diseased fish, which is the same as that of the naturally occurring disease, such as congestion of the ventral and pectoral fin bases, redness and swelling of the anus, and obvious enlargement of the abdomen, etc. An autopsy reveals a large amount of fluid in the abdominal cavity and so on.【Conclusion】 The above results indicated that A. veronii was the causative agent causing this disease, and approved fishery drugs such as neomycin and doxycycline can be used to control the disease during the breeding process. |
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