| 乐翔兆.草地贪夜蛾 bHLH 基因家族鉴定与生物信息学分析[J].广东农业科学,2024,51(8):48-60 |
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草地贪夜蛾 bHLH 基因家族鉴定与生物信息学分析 |
| Genome-wide Identification and Bioinformatics Analysis of bHLH Gene Family in Spodoptera frugiperda |
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| DOI：10.16768/j.issn.1004-874X.2024.08.006 |
| 中文关键词: 草地贪夜蛾 碱性螺旋 - 环 - 螺旋 转录因子 基因家族 功能结构 |
| 英文关键词: Spodoptera frugiperda bHLH transcription factor gene family functional structure |
| 基金项目:江西省自然科学基金（200242BAB20264）；江西省教育厅科学技术研究项目青年项目（GJJ211739） |
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| 中文摘要: |
| 【目的】草地贪夜蛾（Spodoptera frugiperda J. E. Smith）是对我国粮食安全构成巨大威胁的重要入侵
害虫，碱性螺旋 - 环 - 螺旋（Basic helix-loop-helix，bHLH）基因家族转录因子在昆虫生长发育过程发挥重要调控
作用，可作为 RNAi 农药的备选靶标基因。该文目的是确定草地贪夜蛾 bHLH 基因相关靶标，鉴定分析草地贪夜蛾
bHLH 基因家族成员信息。【方法】基于全基因组数据，对草地贪夜蛾 bHLH 基因家族成员的理化性质、亚细胞定位、
蛋白保守结构域、系统发育进化、基因染色体定位、蛋白质三级结构预测等进行分析。【结果】从草地贪夜蛾基因
组中鉴定到 56 个 bHLH 基因，编码 54 条不同的蛋白序列。草地贪夜蛾 bHLH 转录因子氨基酸数目为 84~2557、等
电点为 4.88~10.76、相对分子质量为 9 751.19~285 517.20。草地贪夜蛾 bHLH 基因家族成员分为 A~F 6 组，分别有
25、9、10、1、10、1 个基因，分别编码 24、9、10、1、9、1 条不同的蛋白序列。同组成员有相似类型的 motif，
且同一分组的 bHLH 结构域在系统发育进化分析中大部分归于同一分枝，表明其在进化中具有保守性。草地贪夜蛾
A 组成员 HLH4C（Sfru006728.1）和 B 组成员鸟苷酸环化酶 Gyc76C（Sfru012226.1）被预测包含 bHLH 结构域的同时，
还包含鸟苷酸代谢相关基因的保守结构域，与同组其他基因相比，其三级结构更为复杂，推测两者可能源自草地贪
夜蛾相关的鸟苷酸代谢基因与 bHLH 基因在进化上发生复制与重叠，且可能具有未知的复杂的生理功能。【结论】
该研究分析鉴定到 56 个草地贪夜蛾 bHLH 基因家族成员，获得其序列及理化、亚细胞定位、结构及进化等信息，
有助于进一步研究草地贪夜蛾 bHLH 转录因子家族成员功能及筛选草地贪夜蛾 RNAi 农药靶标。 |
| 英文摘要: |
| 【Objective】Spodoptera frugiperda J. E. Smith is an important invasive pest that poses a great threat to food
security in China. Basic helix-loop-helix (bHLH) gene family transcription factors play important roles in insect growth and
development. Therefore, they may be candidate target genes for RNAi pesticides. In order to determine the relevant targets
of S. frugiperda bHLH genes, the information of S. frugiperda bHLH gene family members was identified and analyzed.
【Method】Based on the genome-wide data, the physicochemical properties, subcellular localization, conserved protein
domain, phylogenetic evolution, gene chromosome localization and tertiary protein structure prediction of the bHLH gene family
members in S. frugiperda were analyzed.【Result】A total of 56 bHLH genes were identified in the genome of S. frugiperda,
encoding 54 different protein sequences. The number of amino acids of SfbHLH transcription factors was 84-2557, the isoelectric point was 4.88-10.76, and the relative molecular weight was 9 751.19-285 517.20. The members of the bHLH gene
family were divided into six groups from A to F, with 25, 9, 10, 1, 10 and 1 genes encoding 24, 9, 10, 1, 9 and 1 different protein
sequences, respectively. Members of the same group had similar types of motif, and most bHLH domains of the same group
were grouped together within the same branch in phylogenetic analysis, indicating that they were conserved in evolution. Group
A member HLH4C (Sfru006728.1) and Group B member guanylate cyclase Gyc76C (Sfru012226.1) were predicted to contain
not only bHLH, but also the conserved domain of genes related to guanylate metabolism, and they had more complex tertiary
structure than other genes in the same group. It was speculated that both of them may be derived from the duplication and
overlap of guanylate metabolism genes and bHLH genes in evolution, and may have unknown complex physiological functions.
【Conclusion】The 56 bHLH gene family members of S. frugiperda were identified and analyzed to obtain their sequence,
physicochemical properties, subcellular location, structure and evolution information, which is helpful to further study the
functions of the bHLH transcription factor family members and screen the RNAi pesticide targets of S. frugiperda. |
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