| 曹华盛,李堂,熊亮,等.水稻磷脂酶D基因家族鉴定及表达分析[J].广东农业科学,2024,(12):- |
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水稻磷脂酶D基因家族鉴定及表达分析 |
| Identification and Expression Analysis of the Phospholipase D GeneFamily in Rice |
| 投稿时间：2024-08-14 修订日期：2024-11-12 |
| DOI： |
| 中文关键词: 水稻 磷脂酶D 基因家族 生物信息学 系统发育分析 表达分析 |
| 英文关键词: Rice Phospholipase D gene family bioinformatics phylogenetic analysis expression analysis |
| 基金项目:广东省基础与应用基础研究基金 (2021A1515010948)；广东省重点领域研发计划 (2020B202090003)；广东省水稻育种新技术重点实验室(2023B1212060042) |
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| 中文摘要: |
| 【目的】磷脂酶D(Phospholipase D, PLD)在植物生长发育、逆境胁迫响应等过程中起着至关重要的作用。挖掘参与水稻(OryzasativaL.)生物和非生物胁迫的 PLD 基因家族成员,探究其生物学功能。【方法】基于水稻基因组数据库鉴定水稻 PLD 家族成员,利用生物信息学的方法系统分析其基因家族成员的蛋白理化性质、系统发育、保守基序、顺式作用元件,通过 RNA-seq 数据分析该家族成员在不同生物和非生物逆境胁迫条件下的表达谱并检测逆境处理条件下总磷脂酶D蛋白活性的变化。【结果】水稻 PLD 基因家族有 17 个成员,氨基酸数目在 512~1115 之间,相对分子质量在 57.19~123.82 kD 之间；蛋白等电点在 5.6~11.23 之间。根据系统进化树将该家族分为 4 个亚家族,不同亚家族成员在外显子数量上存在显著差异；染色体定位分析发现,17 个 PLD 基因定位在 9 条染色体上。顺式作用元件分析发现 PLD 基因家族成员启动子区域含有大量与生长素、脱落酸和茉莉酸甲酯等激素响应及低温响应、压力防御、厌氧诱导响应元件；生物和非生物逆境胁迫条件下 RNA-seq 数据分析发现 PLD 基因家族成员受到了低氮﹑ABA和GA的强烈诱导；此外,总磷脂酶D酶活分析显示高盐胁迫和黑条矮缩病侵染后的磷脂酶D总酶活显著升高。【结论】共鉴定出 17 个水稻 PLD 基因,同亚家族的成员具有类似的保守基序。水稻 PLD 基因家族成员在不同组织及生物和非生物逆境条件下表达存在差异,这些结果将为解析磷脂酶D家族在水稻生长发育﹑生物和非生物逆境胁迫响应中的作用及其机制提供帮助。 |
| 英文摘要: |
| 【Objective】Phospholipase D (PLD) plays a crucial role in plant growth, development, and stress response. Explore the PLD gene family members involved in biotic and abiotic stress in rice (Oryza sativa L.) and investigate their biological functions.【Method】Identifying members of the rice PLD family based on the rice genome database, systematically analyzing the protein physicochemical properties, phylogenetics, conserved motifs, and cis-acting elements of the gene family members using bioinformatics methods. Analyzing the gene expression patterns of the family members under different biotic and abiotic stress conditions through RNA-seq data, and detecting the changes of total phospholipase D activity under stress treatment conditions.【Result】The rice PLD gene family has 17 members with amino acid numbers ranging from 512 to 1115 and relative molecular weights ranging from 57.19 to 123.82 kD; The isoelectric point of these protein are between 5.6 and 11. Between 23. According to the phylogenetic tree, the family is divided into four subfamilies, and there are significant differences in the number of exons among the members in different subfamilies; Chromosome localization analysis revealed that 17 PLD genes were located on 9 chromosomes. The analysis of cis-acting elements revealed that the promoter regions of PLD gene family members contain a large number of hormone responsive elements such as auxin, abscisic acid, and methyl jasmonate, as well as low temperature responsive, pressure defense, and anaerobic induction responsive elements; RNA-seq data analysis under biotic and abiotic stress conditions revealed that members of the PLD gene family were strongly induced by low nitrogen, ABA, and GA; In addition, enzyme activity analysis under biotic and abiotic stress conditions showed a significant increase of total phospholipase D activity after high salt stress and black stripe dwarf disease infection.【Conclusion】A total of 17 rice PLD genes were identified, and members in the same subgroup have similar conserved motifs. The expression of PLD gene family members in rice varies under different tissues and biotic and abiotic stress conditions. These results will provide assistance in understanding the role and mechanism of phospholipase D family in rice growth and development, biotic and abiotic stress response. |
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