| 高洁,相楚嫣,赵俊宏,等.墨兰‘红太阳’不同叶色时期的转录组和代谢组联合分析[J].广东农业科学,2024,(11-12):- |
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墨兰‘红太阳’不同叶色时期的转录组和代谢组联合分析 |
| Joint Transcriptome and Metabolome Analysis of Cymbidium sinense 'Red Sun' at Different Leaf Color Stages |
| 投稿时间：2024-09-03 修订日期：2024-11-06 |
| DOI： |
| 中文关键词: 墨兰 叶色变异 转录组 联合分析 相关性网络分析 |
| 英文关键词: Cymbidium sinense leaf color variation transcriptome joint analysis correlation network analysis |
| 基金项目:国家自然科学基金项目(31902065)；广东省农业科学院科技创新战略专项(高水平农科院建设)(R2021PY-QF003,ZX202402)；广东名优花卉种质资源圃运行维护（2023-NBH-00-009） |
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| 摘要点击次数: 17 |
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| 中文摘要: |
| 【目的】探明‘红太阳’墨兰不同叶色变化过程中相关基因的变化模式，为墨兰叶色改良提供指导。【方法】利用Illumina HiSeq高通量测序平台对‘红太阳’墨兰红色叶片、黄色叶片以及绿色叶片进行测序，分析差异表达基因的富集通路，同时对转录组和代谢组数据进行联合分析，并对筛选出的关键差异基因进行荧光定量PCR的验证。【结果】从红叶向黄叶转变过程中一共筛选到861个差异基因（DEGs），其中上调基因数为204个，下调基因数为657个；从黄叶向绿叶转变过程中一共筛选到815个差异基因，其中上调的基因有492个，下调的基因有323个。KEGG通路富集分析显示与色素合成与代谢相关通路上的基因在‘红太阳’墨兰叶色变化过程中都出现差异显著性的富集。在叶色转变过程中，MYB、NAC、WRKY以及bHLH转录因子家族的表达量出现显著性差异。与代谢组数据联合分析发现，黄酮类、异黄酮类化合物、黄酮和黄酮醇以及代谢物合成途径中的差异基因和差异代谢物在‘红太阳’墨兰叶色变化过程中显著富集。类黄酮代谢途径中共鉴定出26个差异表达基因，这些基因在红叶向黄叶转变过程中均表现出显著下调，5种差异代谢物的含量在这一转变过程中显著下降。在黄叶向绿叶的转变中，17个基因和2种代谢物（新橙皮苷和柚皮苷查尔酮）表现出显著差异。【结论】花青苷合成前体物质含量以及相关合成酶基因表达量的降低导致‘红太阳’墨兰叶片红色逐渐消失，光合色素合成相关基因的高表达是导致‘红太阳’墨兰叶片由黄色最后变绿的主要原因。 |
| 英文摘要: |
| 【Objective】The purpose of this study is to elucidate the patterns of gene changes during the color transition of Cymbidium sinense 'Red Sun', by analyzing the transcriptome of the leaves of Cymbidium sinense 'Red Sun' as they change from red to yellow and finally to green. Concurrently, a joint analysis of transcriptome and metabolome is conducted to provide guidance for the improvement of Cymbidium sinense leaf color.【Method】The method involves using the Illumina HiSeq high-throughput sequencing platform to sequence the red, yellow, and green leaves of Cymbidium sinense 'Red Sun', analyzing the enrichment of differentially expressed genes, and conducting a joint analysis of transcriptome and metabolome data. Key differential genes are also verified by fluorescent quantitative PCR.【Result】The results show that a total of 861 differential genes (DEGs) were screened during the transition from red to yellow leaves, with 204 up-regulated genes and 657 down-regulated genes; during the transition from yellow to green leaves, a total of 815 DGEs were screened, with 492 up-regulated genes and 323 down-regulated genes. KEGG pathway enrichment analysis revealed that genes related to pigment synthesis and metabolism were significantly enriched during the color change process of Cymbidium sinense 'Red Sun'. During the leaf color transition, the expression levels of transcription factor families such as MYB, NAC, WRKY, and bHLH showed significant differences. Joint analysis with metabolome data found that flavonoids, isoflavonoids, flavones, and flavonols, as well as differential genes and metabolites in the metabolic synthesis pathways, were significantly enriched during the color change process of Cymbidium sinense 'Red Sun' A focused analysis was conducted on the differential genes and metabolites involved in the flavonoid metabolic pathway, resulting in the identification of 26 differentially expressed genes, which all showed significant downregulation during the transition from red to yellow leaves. Additionally, the content of five differential metabolites was significantly decreased during this transition. During the transition from yellow to green leaves, 17 genes and two metabolites (naringenin and naringin chalcone) showed significant differences.【Conclusion】The conclusion is that the reduction in the content of anthocyanin synthesis precursors and the expression levels of related synthetic enzyme genes leads to the gradual disappearance of the red color in the leaves of Cymbidium sinense 'Red Sun', and the high expression of photosynthetic pigment synthesis-related genes is the main reason for the leaves of Cymbidium sinense 'Red Sun' changing from yellow to green. |
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