| 霍兴,于咏梅,邱树青,等.高州野生稻重组自交系的构建与穗粒数性状的QTL定位[J].广东农业科学,2024,(11):- |
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高州野生稻重组自交系的构建与穗粒数性状的QTL定位 |
| Construction of Recombinant Inbred Lines of Gaozhou Wild Rice and QTLs Mapping for Grain Number Per Panicle |
| 投稿时间：2024-10-10 修订日期：2024-11-11 |
| DOI： |
| 中文关键词: 野生稻 重组自交系 穗粒数 高密度基因芯片 QTL分析 遗传图谱 |
| 英文关键词: Wild Rice Recombinant Inbred Lines Grain Number per Panicle High-density genotyping arrays QTL Analysis Genetic Map |
| 基金项目:国家重点研发计划“农业生物重要性状形成与环境适应性基础研究”重点专项(2022YFF1002600)；国家自然科学基金面上项目(32071992) |
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| 中文摘要: |
| 【目的】野生稻具有丰富的基因资源，通过构建野生稻重组自交系并进行数量性状基因座（QTL）分析，鉴定控制水稻穗粒数的QTL，进而为水稻遗传改良提供分子基础和遗传资源。【方法】通过高州野生稻和中花11的杂交和多代自交，构建了具有野生稻血缘的重组自交系群体。利用高密度基因芯片对群体进行基因型鉴定，并基于分子标记分析构建了高密度遗传图谱。进一步对群体的穗粒数表型进行了2年的考察，结合表型数据和SNP标记信息，利用QTL IciMapping软件进行了QTL分析。【结果】成功构建了包含316个重组自交系的群体，表型观察显示群体具有丰富的遗传多样性，并在改良穗粒数上有良好的应用潜力。检测到10个调控水稻穗粒数的QTL，其中2022年检测到4个QTL，2023年检测到6个QTL，第11号染色体上的1个QTL表型贡献率最高，为13.78%。位于2号染色体上的QTL在两年中都可以稳定检测到。2年检测到的10个QTL中，有9个加性效应为正值。【结论】成功构建了高州野生稻重组自交系，揭示出高州野生稻在改良水稻穗粒数中的应用潜力，通过QTL分析揭示了控制水稻穗粒数的遗传基础。这些发现为野生稻优异基因的利用和水稻遗传改良提供了理论依据和材料基础。 |
| 英文摘要: |
| 【Objective】 Wild rice possesses a wealth of genetic resources. This study aims to identify quantitative trait loci (QTLs) controlling the grain number per panicle in rice by constructing recombinant inbred lines (RILs) of wild rice and performing QTL analysis. This will provide a molecular basis and genetic resources for the genetic improvement of rice. 【Method】A recombinant inbred line population with wild rice ancestry was developed through crosses between Gaozhu wild rice and Zhonghua 11, followed by multiple generations of selfing. High-density genotyping arrays were used to genotype the population, and a high-density genetic map was constructed based on molecular marker analysis. The phenotypic data for grain number per panicle were examined over two years. QTL analysis was conducted using the QTL IciMapping software, combining phenotypic data and SNP marker information.【Result】A population consisting of 316 recombinant inbred lines was successfully constructed. Phenotypic observations revealed rich genetic diversity within the population and good potential for application in improving the grain number per panicle. Ten QTLS regulating the grain number per panicle in rice were detected, among which four QTLS were detected in 2022 and six QTLS were detected in 2023. The phenotypic contribution of one QTL on chromosome 11 was the highest, accounting for 13.8%. The QTL located on chromosomes 2 was stably detected over two years. The additive effect was positive for 9 out of 10 QTLS detected at 2 years.【Conclusion】The recombinant inbred line of Gaozhou wild rice was successfully constructed, which revealed the application potential of Gaozhou wild rice in improving grain number per panicle of rice. QTL analysis revealed the genetic basis controlling the grain number per panicle in rice. These findings provide a theoretical basis and material foundation for the utilization of excellent genes from wild rice and the genetic improvement of rice. |
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