| 陈谭星,栗焕楠,王品胜,等.构树GRAS基因家族鉴定及其在茎发育中的表达分析[J].广东农业科学,2024,(12-2):- |
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构树GRAS基因家族鉴定及其在茎发育中的表达分析 |
| Identification of GRAS Gene Family in Paper Mulberry and Its Expression Analysis in Stem Development |
| 投稿时间：2024-10-16 修订日期：2024-11-14 |
| DOI： |
| 中文关键词: 构树 GRAS 基因家族 茎发育 木质素 表达分析 |
| 英文关键词: paper mulberry, GRAS, gene family, stem development, lignin, expression analysis |
| 基金项目:河南省科学院基本科研业务费项目(240614033)。 |
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| 摘要点击次数: 26 |
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| 中文摘要: |
| 【目的】GRAS转录因子在植物防御应答、根的发育、分生组织的形成、赤霉素（Gibberellin，GA）信号传导及光信号转导等过程中起重要调控作用，鉴定构树中GRAS基因家族成员并分析其在茎组织中的表达模式，可为研究构树茎木质化发育提供参考。【方法】利用HMM在构树全基因组中鉴定GRAS家族基因，通过生物信息学方法及RNA-Seq数据分析GRAS基因在构树不同木质化程度茎组织的表达模式。【结果】从构树基因组鉴定到43个GRAS家族基因成员，并对它们按染色体分布进行命名。74%的BpGRAS没有内含子，23%的BpGRAS仅含有1~2个内含子。多数BpGRAS蛋白为酸性氨基酸、不稳定蛋白、亲水蛋白，二级结构均以无规则卷曲和α螺旋为主。与拟南芥GRAS蛋白进行系统发育以及蛋白保守结构分析显示，构树的43个GRAS成员被分为9类，且与拟南芥GRAS蛋白具有较高的同源性。顺式作用元件分析显示，该家族可能在植物光反应、响应逆境胁迫及植物体内源激素调控的相关生物学过程中具有潜在功能。基于RNA-Seq的表达分析可知，BpGRAS6、BpGRAS11在T1~T3中连续显著上调，BpGRAS1、BpGRAS15、BpGRAS20、BpGRAS23、BpGRAS29在T1~T3中显著下调。【结论】从构树基因组中成功鉴定出43个GRAS基因家族成员，推测BpGRAS6、BpGRAS11、BpGRAS1、BpGRAS15、BpGRAS20、BpGRAS23、BpGRAS29可能在构树茎木质部形成方面发挥作用，可作为研究构树木质化调控机制的候选基因。 |
| 英文摘要: |
| 【Objective】 GRAS transcription factors play an important regulatory role in plant defense response, root development, meristem formation, gibberellin (GA) signaling and optical signal transduction. Members of the GRAS gene family in paper mulberry were identified and their expression patterns in stem tissues were analyzed. This study can provide a reference for studying the lignification and development of paper mulberry stems.【Method】HMM was used to identify GRAS family genes in the whole genome of paper mulberry, and the expression patterns of GRAS genes in stem tissues with different lignification degrees of paper mulberry were analyzed through bioinformatics methods and RNA-Seq data. 【Result】The genome of paper mulberry was identified to 43 genetic members of the paper mulberry GRAS family, and they were named according to their chromosome distribution.74% of BpGRAS have no introns, and 23% of BpGRAS contain only 1 to 2 introns. Most BpGRAS proteins are acidic amino acids, unstable proteins, and hydrophilic proteins. The secondary structure was dominated by irregular curls and alpha helices. Phylogenetic and protein conserved structure analysis were carried out together with Arabidopsis GRAS protein. The results showed that the 43 GRAS members of paper mulberry were divided into 9 categories and had high homology with Arabidopsis GRAS protein. The analysis of cis-acting elements shows that this family may have potential functions in the related biological processes of plant photoreaction, response to adversity stress, and regulation of plant-derived hormones.Expression analysis based on RNA-Seq showed that BpGRAS6 and BpGRAS11 were continuously significantly upregulated in T1~T3, and BpGRAS1, BpGRAS15, BpGRAS20, BpGRAS23, and BpGRAS29 were significantly downregulated in T1~T3.【Conclusion】In this study, 43 members of the GRAS gene family were identified from the genome of paper mulberry and bioinformatics analysis was performed on them. It was hypothesized that BpGRAS6, BpGRAS11, BpGRAS1, BpGRAS15, BpGRAS20, BpGRAS23, and BpGRAS29 may play a role in the formation of the xylem of paper mulberry stems. It can be used as a candidate gene to study the regulatory mechanism of paper mulberry lignification. This research can provide research reference and theoretical basis for the study of the lignification and development of paper mulberry stems. |
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