| HU Jiaxiao,LIU Jin#,MA Xiaoding,等.利用东乡普通野生稻染色体置换系鉴定抗穗发芽QTL[J].广东农业科学,2024,(11):- |
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利用东乡普通野生稻染色体置换系鉴定抗穗发芽QTL |
| Mapping QTLs for Pre-harvest Sprouting Resistance Using CSSLs of Dongxiang Common Wild Rice (Oryza rufipogon Griff.) |
| 投稿时间：2024-10-25 修订日期：2024-12-04 |
| DOI： |
| 中文关键词: 不同年份环境 东乡普通野生稻 染色体置换系 抗穗发芽 QTL定位 |
| 英文关键词: Different year environments Dongxiang common wild rice CSSLs PHS resistance QTL mapping |
| 基金项目:江西省重大科技研发专项项目(20232ACF01001)、国家重点研发计划项目(2021YFD1200500,2021YFD1200501-7)资助。 |
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| 中文摘要: |
| 【目的】高温阴雨天气导致水稻生产田出现穗上发芽（Pre-harvest Sprouting，PHS），种子活力降低，严重影响水稻产量与品质性状。鉴定筛选抗穗发芽种质及基因资源是培育抗穗发芽水稻新品种、消除稻谷穗上发芽产生危害的根本途径。【方法】本研究以强休眠、不易穗发芽的东乡野生稻C35为供体亲本、较易穗发芽的日本晴（NIP）为受体亲本构建的染色体片段置换系CSSL群体为试验材料，于2021—2023年进行了抗穗发芽特性鉴定评价，以期筛选抗穗发芽种质和鉴定主效QTL。【结果】不同环境下东乡野生稻C35休眠性较强，穗发芽率均为0.00，日本晴存在明显穗发芽现象，穗发芽率均值为31.95%；CSSL群体家系穗发芽率变幅较大，不同年份穗发芽率表型重复性较好，筛选到10份强休眠、抗穗发芽的种质；共检测到14个控制穗发芽率QTL，4个QTL在不同环境下重复被检测到，相关QTL在染色体上形成4个QTL簇qPHSRC1、qPHSRC2、qPHSRC8和qPHSRC9，其中主效QTL簇qPHSRC2和qPHSRC9的LOD值、表型贡献率和加性效应值较大，qPHSRC2为新发现的主效QTL簇。【结论】研究鉴定筛选了一批抗穗发芽的种质材料，定位到14个抗穗发芽QTL，筛选出4个重复性较好的QTL簇，发现一个新的调控穗发芽率的主效QTL簇qPHSRC2，为抗穗发芽目标QTL的精细定位和功能分析奠定了基础。 |
| 英文摘要: |
| 【Objective】High-temperature and rainy weather lead to pre-harvest sprouting (PHS) , severely impacting yield and quality traits in rice. Screening germplasm or genetic resources is a fundamental pathway for developing new varieties resistant to PHS and eliminating PHS damage.?【Method】In this study, a set of chromosome segment substitution line (CSSL) population derived from Dongxiang wild rice (C35) as the donor parent and Nipponbare as the recipient parent were used as the experimental materials, and then PHS resistance were evaluated and QTLs were mapped in 2021-2023 years.【Result】Dongxiang wild rice C35 exhibited strong dormancy across different environments with PHS rate of 0.00%, Nipponbare showed significant PHS with an average PHS rate of 31.95%. The PHS rate varied widely among CSSL population, the phenotypic repeatability was relatively high under different years, meanwhile, ten lines from the CSSL population with strong dormancy and resistance to PHS have been screened. A total of 14 QTLs controlling the PHS were detected, four QTLs repeatedly located. These QTLs formed four QTL clusters (qPHSRC1, qPHSRC2, qPHSRC8 and qPHSRC9), qPHSRC2 and qPHSRC9 had higher LOD values, PVE(%) and add effective, and then qPHSRC2 was a newly discovered major QTL cluster.?【Conclusion】?This research screened a few of PHS resistant germplasms and detected 14 QTLs, four QTL clusters were repeat identified, and then discovered a new major QTL cluster qPHSRC2, which laying the foundation for fine mapping and functional analysis of target QTLs for PHS resistance. |
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