| HU Jiaxiao,LIU Jin#,MA Xiaoding,等.利用东乡普通野生稻染色体置换系鉴定抗穗发芽QTL[J].广东农业科学,2024,(11):- |
| Mapping QTLs for Pre-harvest Sprouting Resistance Using CSSLs of Dongxiang Common Wild Rice (Oryza rufipogon Griff.) |
| 投稿时间：2024-10-25 修订日期：2025-01-14 |
| DOI： |
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| Abstract: |
| 【Objective】High-temperature and rainy weather lead to pre-harvest sprouting (PHS) , severely impacting yield and quality traits in rice. Screening germplasm or genetic resources is a fundamental pathway for developing new varieties resistant to PHS and eliminating PHS damage.?【Method】In this study, a set of chromosome segment substitution line (CSSL) population derived from Dongxiang wild rice (C35) as the donor parent and Nipponbare as the recipient parent were used as the experimental materials, and then PHS resistance were evaluated and QTLs were mapped in 2021-2023 years.【Result】Dongxiang wild rice C35 exhibited strong dormancy across different environments with PHS rate of 0.00%, Nipponbare showed significant PHS with an average PHS rate of 31.95%. The PHS rate varied widely among CSSL population, the phenotypic repeatability was relatively high under different years, meanwhile, ten lines from the CSSL population with strong dormancy and resistance to PHS have been screened. A total of 14 QTLs controlling the PHS were detected, four QTLs repeatedly located. These QTLs formed four QTL clusters (qPHSRC1, qPHSRC2, qPHSRC8 and qPHSRC9), qPHSRC2 and qPHSRC9 had higher LOD values, PVE(%) and add effective, and then qPHSRC2 was a newly discovered major QTL cluster.?【Conclusion】?This research screened a few of PHS resistant germplasms and detected 14 QTLs, four QTL clusters were repeat identified, and then discovered a new major QTL cluster qPHSRC2, which laying the foundation for fine mapping and functional analysis of target QTLs for PHS resistance. |
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