| 罗熹，王涛，卢彪.植物体瞬时表达ChIFN-γ蛋白研究[J].广东农业科学,2013,40(4):128-130 |
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植物体瞬时表达ChIFN-γ蛋白研究 |
| Analysis of transient expression in plants by ChIFN - γ |
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| 中文关键词: |
| 英文关键词: |
| 基金项目:贵州大学研究生创新基金（农科2012009） |
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| 中文摘要: |
| 以生菜、洋葱、萝卜为试材，用转入植物表达载体pSH- NGN的根癌农杆菌EHA105分别采用真空渗透法和直接注射法瞬时表达ChIFN - γ蛋白，研究其最佳宿主植物和最佳方法。结果显示，真空渗透法和直接注射法在瞬时表达效率上差异不大，真空渗透操作上更简便、更易掌握；新鲜市售生菜，真空抽气25 min，共培养3 d，瞬时表达效率达到3645 pg/g，ChIFN - γ蛋白得到高效表达。 |
| 英文摘要: |
| an experiment was conducted to investigate the transient expression mediated by agrobacterium using the leaves of lettuce
(lactuca sativa L.sp), onion (Allium cepa) and radish (Raphanus sativus L.) as host plants and the agrobacterium tumefaciens EHA l05 containing the plant expression vector pSH- NGN by vacuum method and injection method respectively for genetic transformation and detect ChIFN-γprotein expression index to determine the optimal transformation condition. Comparison with injection method, vacuum method was easy and simple to handle, and had the same transient expressing efficiency. When fresh merchant lettuce acted as host, a higher ChIFN - γtransient expression level of 3 645 pg/g could be obtained as vacuum ejection of 25 min and co-culture for 3 days. |
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