| 李廷真，祁俊生，余顺慧，付川.镉对芥菜型油菜萌发生理响应的FTIR研究[J].广东农业科学,2013,40(6):29-32 |
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镉对芥菜型油菜萌发生理响应的FTIR研究 |
| Physiological response of Brassica juncea L. seeds in germination to Cadmium |
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| DOI： |
| 中文关键词: 傅里叶变换红外光谱 芥菜型油菜 镉 发芽率 |
| 英文关键词: FTIR Brassica juncea L. Cd germination percentage |
| 基金项目:三峡库区水环境演变与污染防治重庆高校市级重点实验室开放基金（WEPKL2012MS-01） |
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| 中文摘要: |
| 利用傅里叶变换红外光谱法( FTIR)，对不同Cd2+浓度（0.0、0.001、0.005、0.01 mg/mL）处理条件下萌发14 d的芥菜型油菜幼芽和幼根进行了研究。结果表明，Cd2+处理浓度低于0.001 mg/mL 时对芥菜型油菜种子萌发及生长基本没有影响，甚至对芥菜型油菜种子萌发及生长具有一定的促进作用；但当Cd2+处理浓度达到0.01 mg/mL 时，发芽率、幼根（芽）长等指标都明显下降。在红外光谱研究中，芥菜型油菜根组织在1064、1381、1643、2924、3417 cm-1等处的吸光度呈现先升后降的变化趋势，表明在低浓度Cd2+处理条件下，蛋白质、氨基酸、糖类等物质常作为渗透性调节物质出现，因而致使该类有机物含量增加。但随着Cd2+浓度的增加，该类有机物合成和运输受到限制袁致使其含量逐渐下降。幼芽组织在1072、1404、1635、2924、3433 cm-1等处的吸光度呈现先降后升的变化趋势，这与幼根变化规律相反，说明芥菜型油菜种子在萌发阶段，为了提高根部对Cd2+的耐受性，将幼芽（主要为子叶）中的有机物（如碳水化合物）运输到根部，并将其转化为蛋白质，从而提高对Cd2+的耐受性。 |
| 英文摘要: |
| The physiological response of Brassica juncea L. seeds in germination to cadmium was studied using Fourier transform
infrared (FTIR) spectrometry with different Cd2+ concentrations （0.0, 0.001, 0.005 and 0.01 mg/mL）. The results indicated that, after 14 days stress of Cd, B. juncea L. can grow normally, and no any symptom of Cd toxicity is observed when the Cd concentration is less than 0.001 mg/mL. Compared to the control sample, a lower germination percentage is obtained at the Cd concentration of 0.01 mg/mL. The absorbance of the dominating bands near 1 064, 1 381, 1 643, 2 924, 3 417 cm-1 which corresponded to organic acids, protein amino acids and carbohydrate are initially elevated, and then are decreased in root tissues. With the increase of Cd concentration, the carbohydrate band height of radicle is increased, while a reverse variation trend is obtained for the seedling, indicating that carbohydrate can be transported from seedling to radicle to mitigate the toxic effect of Cd. |
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