| 师尚丽，王庆恒，邓岳文.可口革囊星虫染色体组型分析[J].广东农业科学,2013,40(24):132-133 |
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可口革囊星虫染色体组型分析 |
| Karyotype analysis of Phasolosma esculenta |
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| DOI： |
| 中文关键词: 可口革囊星虫 染色体 组型分析 |
| 英文关键词: Phasolosma esculenta chromosome karyotype analysis |
| 基金项目:广东省科技厅科技计划项目（2012B020307008） |
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| 摘要点击次数: 1568 |
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| 中文摘要: |
| 过滤海水中添加0.04%秋水仙素暂养可口革囊星虫（Phasolosma esculenta）24h后，取星虫体腔液细胞，经低渗、固定、滴片、干燥和染色，制备可口革囊星虫染色体分裂中期相。显微观察、摄影袁根据Levan等的分类标准进行染色体组型分析。结果表明，可口革囊星虫为二倍体，未发现性染色体和随体。染色体基数为x=10，2n=2x=20，染色体组型公式为2n=2x=4m+10sm+6st，NF=40。其中，第1、2、5、6、7对染色体为亚中部着丝粒染色体（sm），第3、10对为中部着丝粒染色体（m），第4、8、9 对为亚端部着丝粒染色体（st）。可口革囊星虫染色体组总长度为40.136 μm，最长染色体为5.129 μm，最短染色体为3.092 μm，平均长度为4.014 μm。臂比值变化范围是1.483~3.566。 |
| 英文摘要: |
| The samples of Phasolosma esculenta were raised for 24 h in 0.04% colchicines solution. Its celomic cells were extracted and treated with lower osmotic pressure, fixed, dropped, air -drying and stained. Photographs of well -spread metaphases were examined and taken with a photomicroscope. The chromosome types were categorized according to Levan. It was found that the basic number of chromosome of P. esculenta was x=10, 2n=2x=20 and the karyotype formula was 2n=2x=4m+10sm+6st, NF=40. The chromosome pair numbers 1, 2, 5, 6, 7 were submetacentric, the chromosome pairs numbers 3, 10 were metacentric, other chromosome pairs were subtelocentric. The total length of genome was 40.136 滋m. In all chromosomes, the longest was 5.129 滋m, the shortest was 3.092 滋m and the average length was 4.014 滋m. Arm ratio was in range of 1.483-3.566. |
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