| 劳水兵1 刘丽辉2 周其峰1 杨玉霞1 马永林1 覃建林1 莫仁甫1.2-甲基-4 氯苯氧乙酸钠在甘蔗茎
和土壤中的残留检测方法[J].广东农业科学,2014,41(1):137-140 |
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2-甲基-4 氯苯氧乙酸钠在甘蔗茎
和土壤中的残留检测方法 |
| Analytic method for determination of MCPA-Naresidue in sugarcane stem and soil |
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| DOI： |
| 中文关键词: 2-甲基-4 氯苯氧乙酸钠 甘蔗 土壤 高效液相色谱 残留 |
| 英文关键词: 2-methyl-4-chlorophenoxyacetic acid sodium (MCPA-Na) sugarcane soil HPLC residue |
| 基金项目:广西自然科学基金（2013GXNSFBA019235），广西
省农科院科技发展基金（桂农科2013JZ07），广西作物病虫害生
物学重点实验室基金（13-051-47-ST-2），广西科学基金（桂科基
0778006-10） |
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| 摘要点击次数: 1743 |
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| 中文摘要: |
| 建立甘蔗茎和土壤中2-甲基-4 氯苯氧乙酸钠残留量的高效液相色谱（HPLC）分析方法，样品经酸性甲
醇水提取，二氯甲烷萃取后，土壤样品以活性炭-硅胶净化，甘蔗茎样品以C18 柱净化，HPLC-UV 检测2 甲4 氯在
0.05~2.00 滋g/mL 范围内线性良好（R2=0.9998），该方法对甘蔗茎和土壤的最低检出浓度均为0.02 mg/kg，添加水平为
0.05，0.10，1.00 mg/kg 时，甘蔗茎回收率为90.86%~93.25%，相对标准偏差为3.31%~4.10%，土壤回收率为84.99%~
89.77%，相对标准偏差为3.42%~8.98%，建立的检测方法分离效果好，回收率准确度和灵敏度高，可用于甘蔗及土
壤中2-甲基-4 氯苯氧乙酸钠的残留量测定。 |
| 英文摘要: |
| An analytical method was developed to determine 2-methyl-4-chlorophenoxyacetic acid sodium (MCPANa)
residue in sugarcane stem and soil by using HPLC. The MCPA-Na residue in sugarcane stem and soil was dissolved in
acidic methanol and extracted by dichloromethane, the soil sample was purified through activated carbon-silica gel, while
the sugarcane stem sample was purified through C18 column, then the samples were detected by HPLC-UV. This method
could be used to determine the MCPA-Na residue in sugarcane and soil with good linear relationship 0.9998 when the
concentration range of MCPA was 0.05~2.00 mg/L and both the limits of detection were 0.02 mg/kg. The recovery rates of
MCPA-Na from sugarcane stem and soil fortified with MCPA at 0.05, 0.10, 1.00 mg/kg were 90.86%~93.25% and 84.99%
~89.77% respectively, with relative standard deviation in the range of 3.31%~8.98豫. The developed method, which had
good separating effect, high recovery rate, accurancy and sensitivity, could be used to detect the MCPA-Na residue in
sugarcane and soi1. |
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