| 惠俊爱.镉胁迫对超甜玉米CT38叶片DNA 交联的影响[J].广东农业科学,2014,41(2):5-8 |
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镉胁迫对超甜玉米CT38叶片DNA 交联的影响 |
| Effects of cadmium stress on DNA cross-linkingof super sweet corn CT38 leaves |
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| DOI： |
| 中文关键词: 玉米 DNA 交联 |
| 英文关键词: cadmium corn DNA cross-linking |
| 基金项目:国家自然科学基金重点项目（40730741）国家；863冶
计划项目（2007AA0610-01）广东省自然科学基金团队项目
（9351064101000001）；华南理工大学自然科学基金（E5090550) |
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| 中文摘要: |
| 研究了外源镉胁迫对盆栽玉米叶片DNA 交联的影响，结果表明，随着镉处理浓度的增加，超甜玉米
CT38 叶片DNA 的提取量下降，镉处理10 d后DNA 的提取量在镉处理浓度为1 100 mg/kg 下与同期CK 相比分别
下降了8%，52%镉胁迫降低了玉米叶片中DNA 的提取量且胁迫时间越长对植物DNA 的损伤越严重；随着镉胁迫
浓度的增加玉米叶片DNA 增色效应呈先上升后下降的规律；镉胁迫下玉米叶片DNA 电泳条带明显向低分子量漂
移处理30，60 d时在镉浓度5~100 mg/kg 范围内小分子DNA 含量随着镉浓度的增加而增加。 |
| 英文摘要: |
| Effects of exogenous cadmium on DNA cross-linking in leaves of potted corn were studied. Results showed
that as the cadmium concentration increased, the extraction of DNA in leaves of super sweet corn CT38 decreased. 10 d
after the treatment with cadmium, DNA extraction in the treatment of cadmium concentration in 1 and 100 mg/kg decreased
8% and 52% respectively, compared with CK in the same period. Cadmium reduced the extraction of DNA in corn leaves,
and the longer the stress on DNA, the serious damage the plants suffered. With the increasing cadmium concentrations,
corn DNA hyperchromic effect increased first then decreased. Electrophoresis bands of leaves DNA under cadmium stress
dirfted to low-molecular-weight obviously. With the processing of 30 and 60 d, under cadmium concentrations in range of
5~100 mg/kg, DNA content of small molecule increased as the cadmium concentration increased. |
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