| 褚夫江1, 2，刘文彬1, 2，吴玉萍1, 2，金小宝1, 2，朱家勇1, 2.不同提取方法对家蝇幼虫体外抗炎活性的影响[J].广东农业科学,2014,41(4):118-121 |
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不同提取方法对家蝇幼虫体外抗炎活性的影响 |
| Effects on the in vitro anti-inflammatory activity ofextraction products of housefly (Musca Domestica)maggot obtained by different methods |
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| DOI： |
| 中文关键词: 家蝇幼虫 抗炎 提取方法 |
| 英文关键词: housefly (Musca Domestica) maggot anti-inflammatory extraction method |
| 基金项目:国家自然科学基金（81274061）；广东省战略性新
兴产业核心技术攻关项目（2012A080800016）；广东省医学科研
基金（B2013189） |
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| 摘要点击次数: 1149 |
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| 中文摘要: |
| 为比较水提法、碱提法、盐提法及醇提法对家蝇幼虫体外抗炎活性的影响，以脂多糖诱导的巨噬细胞为
研究对象，结合不同方法所获产物的产率、蛋白含量等，探明4 种方法所获提取物对细胞形态及TNF 琢、MCP-1 分泌
的影响。结果表明，碱提法所获产物的产率最高，其次是水提法、盐提法和醇提法；水提法所获产物的蛋白含量最高，
其次是盐提法、碱提法和醇提法；水提法所获产物的体外抗炎活性最优，其次是醇提法、盐提法和碱提法。 |
| 英文摘要: |
| In order to compare the anti-inflammatory activity effects of the extraction products of housefly maggot
obtained by the extraction methods of water, alkali, salt and alcohol, the macrophages which induced by LPS were used in this
experiment. The yields and protein content was tested and the effects on the cell morphology and secretion of TNF 琢, MCP-1
were detected as well. The results showed that the yield of the alkali solution extraction was higher than other methods,
followed by the water extraction, salt solution extraction and alcohol extraction. However, the protein content of water
extraction was the highest, and the salt solution extraction, alkali solution extraction and alcohol extraction followed. The
results also showed that the product of water extraction had the function of anti-inflammatory and was more significant than
other methods, the alcohol extraction, salt solution extraction and alkali solution extraction followed. |
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