| 王萱1、2、吴波2、程春振2、姜波2、钟广炎2.甜橙乙烯诱导酯酶基因家族的进化[J].广东农业科学,2014,41(10):135-145 |
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甜橙乙烯诱导酯酶基因家族的进化 |
| Evolution of Ethylene-induced esterase genefamily members in Citrus sinensis |
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| DOI： |
| 中文关键词: 甜橙 乙烯诱导酯酶 家族基因 进化 |
| 英文关键词: Citrus sinensis Ethylene-induced esterase gene family evolution |
| 基金项目:基金项目院国家国际科技合作专项（2012DFA30610） |
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| 中文摘要: |
| 为了解甜橙乙烯诱导酯酶基因（Citrus sinensis Ethylene-induced esterase gene、CsEIE）家族成员的进化历
史与功能分化、检索比对了甜橙基因组尧TAIR 数据库尧Phytozome 数据库和NCBI EST 数据库中多个物种的EIE 家族
基因、分析了甜橙中该基因家族的全部18 个成员的基因结构尧染色体分布尧蛋白结构域尧保守序列尧基因表达尧进化
选择方向以及系统发生关系。结果表明、CsEIE 家族成员分布在4 条染色体中、通常呈串联重复排列；不同基因的外
显子数目变异范围为1~5；该基因家族绝大多数成员含有3 个保守基序。系统发生学分析结果显示、被子植物EIE 家
族基因聚为3 个分支、表明EIE 基因家族是在被子植物分支前就已存在的古老的多基因家族、家族成员目前的分布
现状是基因不断重复和丢失的结果。系统发生树中、甜橙CsEIE1~CsEIE8 属于同一分支、推测其是在柑桔与研究中
其他物种分化后经多次基因重复事件产生。该分支在其他被子植物中也同样有多个经独立基因重复事件形成的旁
系同源、而另外两支中发生的重复事件要少得多。表达分析结果显示、CsEIE 基因家族在转录活性尧表达模式上已经
出现了较大的分化。所有结果表明CsEIE 基因家族仍在扩张、各成员在经受不同的选择压力。 |
| 英文摘要: |
| The Citrus sinensis Ethylene -induced esterase genes (CsEIE) were studied to reveal their family爷s
evolutionary history and their phylogentic realtionships. A total of 18 CsEIE gene family members were identified by
analyzing the C. sinensis genome, and their homologs were also identified from several other plant species through
searching TAIR, Phytozome and NCBI EST databases. The 18 members were comparatively analyzed for their gene
structures, relative locations on chromosomes, protein domains and conserved regions, gene expression patterns,
evolutionary direction and phylogenetic relationships. Results showed that the CsEIE genes were located on four different
chromosomes, mostly as tandem repeats. The number of exons of the members varied from 1 to 5. Most of the members
possessed 3 conserved motifs. Phylogenetic trees showed that EIE gene family members were clustered into three branches
which were also prsenent in all analysed angiosperms, suggesting that EIE gene family is an ancient multigene family
existing before the branching of angiosperms, and the current status of this gene family should be a result of gene
duplication and gene differentiation. In the phylogenetic tree, CsEIE1 -CsEIE8 were clustered together independently,
indicating they were derived from other species through gene duplication events after divergence from their common
ancestor. Expression analysis showed that the transcriptional activity and expression patterns of CsEIE gene family were
greately different. All the results indicte that the CsEIE gene family is still expanding and the members are experiencing
different selection pressures |
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