| 刘素英，赵孟良，王丽慧，孙雪梅，李屹，李莉.干旱胁迫下菊芋蔗糖磷酸合成酶及转化酶活性变化规律研究[J].广东农业科学,2014,41(16):23-28 |
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干旱胁迫下菊芋蔗糖磷酸合成酶及转化酶活性变化规律研究 |
| Research on change rule of sucrose phosphate synthase and invertase activities in Helianthus tuberosus Linn under drought stress |
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| DOI： |
| 中文关键词: 菊芋 干旱胁迫 蔗糖磷酸合成酶（SPS） 蔗糖转化酶（INN） 酶活性 |
| 英文关键词: Helianthus tuberosus Linn drought stress sucrose physphate synthase (SPS) invertase (INV) enzyme activity |
| 基金项目:农业部“948”项目（2013-Z72）；青海省科技厅应用基础研究项目（2012-H-809） |
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| 中文摘要: |
| 为研究干旱胁迫下不同菊芋品种不同部位的蔗糖磷酸合成酶（SPS）及蔗糖转化酶（INV）的活性变化规律，采用PEG模拟与人工控水胁迫的方式对青芋1号、青芋2号两个菊芋品种进行研究。结果表明院（1）青芋1号和青芋2号在10%、20%PEG胁迫下，茎中SPS活性均较对照高，而30%PEG胁迫下茎中SPS活性较对照低，INV活性均表现较低；而不同浓度PEG胁迫处理的叶片中，SPS活性均较对照高，INV活性表现仍然较低遥（2）青芋1号和青芋2号茎、叶中的SPS活性在不同控水强度胁迫前期均较对照高；青芋1号INV活性在中度控水35d时活性较低、蔗糖合成增加，青芋2号INV活性在所有控水条件下7 d后均下降，故适宜的控水强度及控水时间可以提高青芋1号和青芋2号茎、叶中的蔗糖含量。 |
| 英文摘要: |
| In order to study the change rule of sucrose phosphate synthase (SPS) and invertase (INV) activities in different varieties and different parts of Helianthus tuberosus Linn under drought stress, the trials were carried out with two kinds of H. tuberosus including Qingyu1 and Qingyu2 through the way of PEG simulation with the water stress control. The results showed that: (1) the SPS activities in stems of Qingyu1 and Qingyu2 were higher than that of CK under 10% and 20% PEG stress, but lower that under 30% PEG stress, and the INV activities in stems were relatively low under different concentrations of PEG stress. The SPS activities in leaves were higher than that of CK and the INV activities were still low under different concentrations of PEG stress. (2) The SPS activities in stems and leaves of Qingyu1 and Qingyu2 were higher than those of CK at the early stage of different degrees water stress control. The INV activity of Qingyu1 was relatively low on the 35th day under moderate water stress control, which increased the sucrose synthesis, and the INV activity of Qingyu2 declined on the 7th day under different degrees of water stress control. So the appropriate intensity and
duration of water stress control could improve the sucrose content in the stems and leaves of Qingyu1 and Qingyu2. |
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