| 韩春艳，郑清梅，陈桂丹，刘丽霞.不同投喂模式对奥尼罗非鱼生体内脂类代谢的影响[J].广东农业科学,2014,41(16):120-124 |
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不同投喂模式对奥尼罗非鱼生体内脂类代谢的影响 |
| Effects of feeding regimes on growth performance,lipid metabolism of juvenile hybrid tilapia(Oreochromis niloticus伊O. aureus) |
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| DOI： |
| 中文关键词: 奥尼罗非鱼 投喂模式 补偿生长 脂类代谢 |
| 英文关键词: Oreochromis niloticus×O. aureus feeding regime compensatory growth lipid metabolism |
| 基金项目:广东省自然科学基金（S201101000345，S20130100136931）；嘉应学院自然科学研究重点项目（2011KJZ05）；广东省优秀青年教师计划项目（Yq2013152） |
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| 中文摘要: |
| 研究了多重循环野饥饿-投喂冶模式对体质量约6.8 g的奥尼罗非鱼（Oreochromis niloticus）O.aureus（幼鱼补偿生长、体组成和肝脏内苹果酸脱氢酶（MDH）、激素敏感性脂肪酶渊HSL（活力及mRNA表达的影响。试验鱼分为4组：对照组（CG，每日投喂）、试验1组（G1，每周投喂6d）、试验2组（G2）每周投喂5d、和试验3组（G3，每周投喂4d），试验周期为56d。结果显示：G1出现完全补偿生长，其终末体质量尧增重率及特定生长率等与对照组差异均不显著；G1和G2的饲料效率显著提高；随周期饥饿时间的延长袁鱼体水分和灰分含量显著增加，而蛋白质和脂肪含量显著下降；随周期饥饿时间的延长袁MDH的活力和mRNA表达明显下降，HSL的活力和mRNA表达则明显提高，恢复投喂后二者的活力和mRNA表达快速恢复，但均高于对照组。结果表明，饥饿1d、连续投喂6d的投喂模式下，奥尼罗非鱼可通过提高饲料的转化率来达到完全补偿生长。同时，在循环饥饿条件下，奥尼罗非鱼可通过改变体内与脂类代谢相关酶的活性及mRNA表达为机体储备能量物质。 |
| 英文摘要: |
| To determine the effects of feeding regimes on growth performance and lipid metabolic enzymes in liver of juvenile hybrid tilapia (Oreochromis niloticus×O. aureus),180 fishes with similar body weight (6.80依0.50 g) were randoml divided into 4 groups, including control group (CG, 7 days/week of feeding), group 1 (G1, 6 days/week of feeding), group 2 (G2, 5 days/week of feeding), and group 3 (G3, 4 days/week of feeding). At the end of 56 d experiment, growth performance and the activities and mRNA expression of malic dehydrogenase (MDH) and hormone-sensitive lipase (HSL) in liver were measured. Results showed that, fishes in G1 group got full compensatory growth. There was no significant difference between G1 and CG in final weight, weight gain rate and specific growth rate. Feed efficiency in G1 and G2 significantly increased. With the starvation time prolonging, the moisture and ash content of fish increased significantly, the protein and fat content significantly decreased, MDH activity and mRNA expression in liver of fish significantly decreased, HSL activity and mRNA expression significantly increased. After reefed, the activity and mRNA of MDH, HSL fast recovered, but the values were higher than those in CG. The results indicate that tilapia can improve the feed conversion rate to achieve full compensation growth in 6 days/week of feeding regime. At the same time, in the cycle starvation-refeed regime, tilapia can
change the activity and mRNA expression of lipid metabolism enzymes to reserve energy substances. |
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