| 徐港明，孙海燕,顾雯雯，王淑园，罗兵，杨志刚，沈宗根，端木银熙.太湖地区24 份杂交粳稻DNA指纹图谱的构建及遗传相似性分析[J].广东农业科学,2014,41(20):1-4 |
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太湖地区24 份杂交粳稻DNA指纹图谱的构建及遗传相似性分析 |
| DNA fingerprint establishment and genetic similarity analysis of japonica hybrid rice in Taihu area |
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| DOI： |
| 中文关键词: 杂交粳稻 DNA指纹图谱 遗传相似性 太湖地区 |
| 英文关键词: japonica hybrid rice DNA fingerprint genetic similarity Taihu area |
| 基金项目:江苏省农业科技支撑计划项目（BE201144);苏州市科技计划项目(SZS201102,SYN201110,SNG201323);江苏省大学生实践创新训练计划项目（2013035）；苏州市吴江区科技计划项目（WN201311，WN201317）；常熟市科技项目（N201409，CS201205） |
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| 中文摘要: |
| 利用SSR 标记对24 份太湖地区杂交粳稻品种初步建立了DNA 指纹图谱和进行遗传相似性分析。结果表明：24对引物在研究材料中共检测到105 个多态性片段，平均每对引物的多态性片段为4.38 个。筛选出5对核心引物（RM336、RM72、RM1195、RM19 和RM267）建立了24 份材料的DNA指纹图谱。24份材料之间的相似系数变化范围为0.69~0.99，相似系数比较大，表明供试材料的遗传基础多样性相对狭窄。在遗传相似系数0.71 水平处可以将24 份材料分为3个类群。 |
| 英文摘要: |
| SSR was used to construct the DNA fingerprint and analyze the genetic similarity of 24 japonica hybrid rice in Taihu area. The results showed that 24 SSR primers amplified a total of 105 polymorphic DNA bands, averaging 4.38 bands per primer. In this study, 5 pairs of core SSR makers (RM336, RM72, RM1195, RM19 and RM267) were screened among 24 pairs of SSR primers to establish DNA fingerprint of the japonica hybrid rice. The result of cluster analysis showed that genetic similarities among the 24 lines ranged from 0.69 to 0.99. The values of similar coefficient were rather high, which indicated that genetic diversity of japonica hybrid rice in Taihu area was correspondingly narrow. The cluster analysis also showed that 24 japonica hybrid rice could be divided into three groups at 0.71 of the similar coefficient. |
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