| 曾超珍,刘志祥,董旭杰.板栗壳抑菌物质提取工艺的优化[J].广东农业科学,2014,41(21):102-105 |
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板栗壳抑菌物质提取工艺的优化 |
| Optimization on the extraction technology for antibacterial substances in chestnut shells |
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| DOI： |
| 中文关键词: 板栗壳 正交试验 抑菌物质 |
| 英文关键词: chestnut shell orthogonal experiment antibacterial substance |
| 基金项目:中南林业科技大学青年科学基金（QJ2011042B） |
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| 摘要点击次数: 1412 |
| 全文下载次数: 641 |
| 中文摘要: |
| 采用单因素和正交试验探讨板栗壳抑菌物质的最佳提取工艺，并研究板栗壳抑菌物质的体外抗菌作用以及pH值和温度对其抗菌活性的影响。结果表明：板栗壳抑菌物质的最佳提取工艺为提取溶剂为乙酸乙酯，浸提时间4.0h，提取温度70℃，料液比1：30；提取物对大肠杆菌、金色葡萄球菌、枯草杆菌均具有抑菌效果；最低抑菌浓度分别0.125、0.0625、0.125mg/mL；最小杀菌浓度分别为0.125、0.125、0.25 mg/mL。抗菌活性有较强的热稳定性，能耐短时的高温处理，在pH 值7~9 的范围具有较强的抗菌活性。 |
| 英文摘要: |
| In this paper, the optimum extractive technology of bacteriostasis from chestnut shells and its antibacterial
activity were studied by using single factor experiment and orthogonal experiment, and then the bacteriostatic activity of
chestnut shell extracts and the effects of temperature, pH value on it were studied by cylinder plate method. The results
showed that optimum extractive technology of bacteriostasis from chestnut shells were as follows: ethyl acetate as solvent, extracting time 4.0 h, extracting temperature 70℃, and ratio of solid to liquid 1：30. The substance extracted showed some antibiotic activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and the minimum inhibitory concentrations (MIC) were 0.125, 0.0625, 0.125 mg/mL, respectively, and the minimum bactericidal concentrations (MBC) were 0.125, 0.125, 0.25 mg/mL respectively. It could meet the moment heat treatment and had strong antimicrobial activity within pH 7-9. |
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