| 柳凤1,2，欧雄常1，韦继光2，詹儒林1，常金梅1.应用ISSR 技术分析杧果畸形病
病原菌的遗传多样性[J].广东农业科学,2014,41(24):79-82 |
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应用ISSR 技术分析杧果畸形病
病原菌的遗传多样性 |
| Study on genetic diversity of Fusarium mangiferae frommango malformation disease by ISSR analysis |
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| DOI： |
| 中文关键词: 杧果畸形病 ISSR 遗传多样性 |
| 英文关键词: mango malformation disease interal simple sequence repeat genetic diversity |
| 基金项目:中央级非盈利性研究机构基本科研业务费研究专
项（1630062014007）；农业行业科研专项(201203092-3) ；四川省
科技计划项目（014NZ0026） |
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| 摘要点击次数: 1347 |
| 全文下载次数: 444 |
| 中文摘要: |
| 为探索我国杧果畸形病病原菌的遗传多样性，运用ISSR 分子标记技术分析了采自四川攀枝花市和云
南华坪县的38 个杧果畸形病病原菌的遗传多样性。供试的38 个菌株经14 条特异性引物共扩增出72 条带，其中52
条带具有多态性，多态性比率为72.22%。38 个菌株的相似系数值为0.5972~0.9862，变幅为0.3889，平均为0.7885。其
中菌株MG16 与MG17 的相似系数最大、为0.9862曰菌株MG07 和MG33 的遗传相似系数最小、为0.5972。利用
UPGMA 法进行聚类分析，38 株菌株在相似系数为0.76 水平上分为5 类，菌株MG33、MG07 和MG36 分别形成单一
分支，MG25 和MG35 聚合在一起曰其余菌株分布在第吁类中。我国杧果畸形病病原菌Fusarium mangiferae 存在丰富
的种资资源，且F. mangiferae 与采集地点、杧果品种之间无明显相关性。 |
| 英文摘要: |
| In order to explicit the genetic diversity of 38 strains Fusarium mangiferae from mango malformation
disease from Panzhihua, Sichuan, and Huaping, Yunnan in China, the ISSR technique was used. 72 bands were amplified
by 14 ISSR primers, with an average of 5.14 bands per primer. The size of the amplified products ranged from 300 bp to
2 000 bp. The genetic similarities among isolates ranged from 0.5972 to 0.9862. Isolates MG16 and MG17 were the most
similar(0.9862 genetic similarity), whereas isolates MG07 and MG33 were the least similar(0.5972) and were grouped in
separate clusters. At a genetic similarity of 0.76, the unweighted pair -group method with arithmetic mean analysis
separated the isolates into five distinct clusters, comprising 33, two, one, one and one isolate, respectively. The ISSR data
supported the conclusion that the isolates of F. mangiferae were not necessarily related by geographic origin, type of tissue
or the mango cultivar from which they were isolated. This is the first report on the diversity of F. mangiferae species
associated with mango malformation in China. |
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