| 李维1，谢海强1，刘若余1，林家栋1，李思1，杜雪琴2，肖超能1.家兔UCP1 第3外显子基因多态性研究[J].广东农业科学,2014,41(24):138-141 |
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家兔UCP1 第3外显子基因多态性研究 |
| Study on the SNP of UCP1 Exon3 in rabbits |
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| DOI： |
| 中文关键词: UCP1 比利时兔 新西兰兔 加利福尼亚兔 多态性 |
| 英文关键词: UCP1 Belgian rabbit New Zealand rabbit California rabbit polymorphism |
| 基金项目:贵州省科技支撑计划项目（黔科合NY 字[2012]
3013 号） |
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| 摘要点击次数: 1387 |
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| 中文摘要: |
| 为了解兔肉质性状特征，给兔的品种选种选育提供理论依据，选择UCP1 基因设计1 对引物扩增其第3
外显子及第2 内含子部分序列，采用DNA 池PCR 反应，直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3
个兔品种UCP基因多态性。结果在新西兰兔中发现1 个多态性位点（T387C），多态位点对UCP1 基因的RNA 结构产
生影响，其最小自由能由-917.51 kJ/mol 变为-927.12 kJ/mol，而其他2 个品种没有检测到该突变。 |
| 英文摘要: |
| In order to deeply understand the meat quality and provide basic information and scientific proof for better
heredity selection of rabbit, the complete sequences of exon3 and part of intron2 region in UCP1 of rabbit were obtained by
designing one pair of specific primers. DNA pools PCR and the direct sequencing were used to test the SNP site in Belgian
rabbit, New Zealand rabbit and California rabbit. The results showed that only one SNP (G387C) site was found in New
Zealand rabbit, and the polymorphism led to the changes of RNA secondary structure, its minimum free energy changed
from -917.51 kJ/mol to -927.12 kJ/mol. However, there was no SNP site in the other two breeds. |
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