| 谢 静， 钟艳霞， 罗玲玲，等.黄河湿地降解菌的分离鉴定及系统发育树的构建[J].广东农业科学,2015,42(3):153-157 |
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黄河湿地降解菌的分离鉴定及系统发育树的构建 |
| Isolation, identification and construction of phylogenetic tree of degrading bacteria in Yellow River Wetland |
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| DOI： |
| 中文关键词: 降解菌 16S rRNA 系统发育树 黄河湿地 |
| 英文关键词: degrading bacteria 16S rRNA phylogenetic tree Yellow River Wetland |
| 基金项目: |
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| 摘要点击次数: 1403 |
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| 中文摘要: |
| 采用富集培养法分离提纯出了 6 株降解菌，通过研究其降解能力，筛选出降解效果较高的两株菌
H3 和 H6， 利用 16S rRNA 基因克隆法鉴定其基因序列， 同时将基因序列与 NCBI 数据库中已有细菌的 16S
rRNA 序列进行相似性比较分析后构建系统发育树， 最后结合菌落的形态学特征和生理生化特征确定其生物
学地位。 结果表明，6 株菌对污染物均有一定的降解能力， 而且对 CODcr 的降解效果要优于对 TP 和 NH3-N
的； 菌株 H3 和 H6 对 CODcr、TP、NH3-N 的去除率分别为 50.2%、26.83%、44.89%及 52.19%、25.16%、46.79%，降
解能力较突出；菌株 H3 与荧光假单胞菌 Pseudomonas fluorescens （NR_113647.1）亲缘性最近，同源性达 99%；
菌株 H6 与解淀粉芽孢杆菌 Bacillus amyloliquefaciens（NR_075005.1）同源性达 99%。分离所得到的荧光假单胞
菌和解淀粉芽孢杆菌对生活污水具有一定的净化作用，且安全性有保障，可以应用于湖泊湿地水体的生态修
复和生活污水处理中。 |
| 英文摘要: |
| Enrichment culture method was adopted to separate and purify 6 bacteria which had degradative
capability. Two strains H3 and H6 with higher degradative capability were screened by studying their degradation
ability. 16S rRNA gene clone method was adopted to identify their gene sequences and a phylogeny tree was
constructed after a comparative analysis on similarity with the existing bacterias 16S rRNA gene sequences in the
National Center for Biotechnology Information. Then, combined with their morphological, physiological and
biochemical characteristics, this study determined their biological status. The results showed that 6 bacteria had
certain ability to degrade pollutants, and the removal efficiencies of CODcr were better than those of TP and NH3-N;
H3爷s removal rates of CODcr, TP, NH3-N were 50.2%, 26.83% and 44.89%, H6s removal rates of CODcr, TP, NH3-
N were 52.19%, 25.16% and 46.79%, their degradation abilities were more prominent. H3 and Pseudomonas
fluorescent (NR_113647.1) had very close affinity, their homology could up to 99%; the homology of H6 and Bacillus
amyloliquefaciens (NR_075005.1) was 99%. The P. fluorescent and B. amyloliquefaciens isolated from Yellow River
Wetland have purifying effect on domestic sewage, and their security is guaranteed, they can be applied to ecological
restoration of lake wetlands and sewage treatment |
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