| 曹宗喜，郑心力，张 艳，等.PCV2海南株全基因组的克隆与序列分析[J].广东农业科学,2015,42(8):113-123 |
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PCV2海南株全基因组的克隆与序列分析 |
| Cloning and sequence analysis of complete genome ofporcine circovirus isolated from Hainan province |
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| DOI： |
| 中文关键词: 猪圆环病毒2型 全基因组 克隆 序列分析 |
| 英文关键词: porcine circovirus type 2 complete genome cloning sequence analysis |
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| 中文摘要: |
| 为了研究猪圆环病毒 2 型（PCV2）海南株的分子特征，采用PCR 方法从疑似 PCV2 感染的猪组织
病料中扩增基因并进行序列分析。结果表明8 株 PCV2 海南株均属于 PCV2b 型，其中7 株为PCV2b-1C亚型，
1株为PCV2b-1A/1B亚型。PCV2 海南株的ORF2 基因的长度均为705…bp，编码234 个氨基酸。Cap蛋白的抗原表
位区发生了一定的变异。8个 PCV2 海南株的ORF2 基因之间核苷酸序列相似性及推导的氨基酸序列相似性分别
为95.3%~99.7%和93.6%~100%。海南株与国内分离株（AY682994，AF381175，JX945577，JX682407，AY180397）
的核苷酸序列相似性为91.0%~99.9%，推导的氨基酸相似性为91.0%~99.6%。海南株与国外分离株（NC_005148，
JQ994268，KJ187306，AF201307，AF454546，AY754020）的核苷酸序列相似性为 90.0%~97.0%，推导的氨基酸
相似性为 88.0%~97.9%。海南株与疫苗株 SH的核苷酸序列相似性为 98.2%~100.0%，推导的氨基酸相似性为
94.9%~100.0%。海南株与疫苗株LG的核苷酸序列相似性为90.6%~91.7%，推导的氨基酸相似性为89.7%~91.0%。
这些数据为海南地区PCV2的防控和疫苗株选择提供了理论依据。 |
| 英文摘要: |
| To understand the molecular characteristics of PCV2 isolated from Hainan province，the completegenome of PCV2 of some PMWS-suspected samples from Hainan province were amplified sequence analysis wascarried out. The results showed that a total of 8 PCV2 strains was isolated and identified，and all the isolates belongedto genotype 2b，of which 7 isolates were grouped into cluster PCV2b-1C and 1 isolate was grouped into cluster PCV2b-1A/1B. The ORF2 gene of 8 PCV2 strains were 705 bp，encoding 234 amino acids. The main antigen epitope areachanged significantly in Cap protein of PCV2. The identity of PCV2 ORF2 sequence among 8 isolates was 95.3%-99.7%of nucleotide similarity，and 93.6%-100% of deduced amino acid similarity. They had 91.0%-99.9% nucleotide similarityand 91.0%-99.6% deduced amino acid similarity with AY682994，AF381175，JX945577，JX682407 and AY180397；90.0%-97.0% nucleotide similarity and 88.0%-97.9% deduced amino acid similarity with NC_005148，JQ994268，KJ187306，AF201307，AF454546 and AY754020；98.2%-100.0% nucleotide similarity and 94.9%-100.0% deducedamino acid similarity with SH；90.6%-91.7% nucleotide similarity and 89.7%-91.0% deduced amino acid similarity withLG. This study got the basic epidemiological data for the prevention and control of PCV-2 in Hainan Province，it can alsohelp the choice of PCV-2 vaccine. |
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