| 冯爱娟1，2，吴酬飞3，叶 茂1，2，邓毛程1，2.大孔树脂吸附法分离纯化竹叶黄酮研究[J].广东农业科学,2015,42(19):89-94 |
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大孔树脂吸附法分离纯化竹叶黄酮研究 |
| Separation and purification of flavonoids frombamboo leaves with macroporous resin |
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| DOI： |
| 中文关键词: 大孔吸附树脂 竹叶黄酮 吸附 纯化 动力学 |
| 英文关键词: macroporous resin flavonoids from bamboo leaves absorption purification kinetics |
| 基金项目:广东高校特色调味品工程技术开发中心建
设项目（GCZX-B1103）；广东轻工职业技术学院校级项
目（KJ201107）；广东省高等职业教育专业领军人才培育
项目；“创新强效工程”自主创新能力提升项目 |
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| 摘要点击次数: 1342 |
| 全文下载次数: 539 |
| 中文摘要: |
| 采用大孔树脂吸附分离的方法对粉单竹竹叶黄酮提取物进行纯化，探讨D101 大孔吸附树脂的静
态及动态吸附解吸动力学特性，并对树脂动态柱层析的工艺条件进行优化。结果表明，D101 大孔树脂较适宜
于竹叶黄酮提取物的纯化；动态柱层析的工艺条件为：上样溶液的pH 为8.0，上样流速1.0 mL/min，分别用2
倍柱床体积的20%、40%、60% 及80% 乙醇以1.5 mL/min 的洗脱速率进行阶梯梯度洗脱。在优化工艺条件下，
可以收集得到纯度分别为50.9%、38.0%、35.8% 等3 个竹叶黄酮产品，黄酮总回收率可达63% 左右。该工艺
既可满足产品高纯度的要求，又保证了竹叶黄酮的高回收利用率，具有可行性。 |
| 英文摘要: |
| This paper used macroporous resin to purify flavonoids from bamboo leaves，discussed the kinetics
of absorption and desorption of static and dynamic of macroporous resin to flavonoids，and optimized the process
of resin column chromatography dynamic as well. The results showed that D101 macroporous resin was suitable for
purification of flavonoids from bamboo leaves；the optimum process conditions were as follows：pH 8.0，flow rate of
loading sample at 1 mL/min，the solvents for adsorption 20%，40%，60% and 80% ethanol respectively with 2 BV at
the flow rate of 1.5 mL/min. Using the optimized purification process，three flavonoids products with 50.9%，38.0%
and 35.8% purity could be obtained，and the total recovery rate of flavonoids was 63%. This process can meet the
requirements of high product purity，and ensure the high utilization rate of recycling of bamboo leaves flavonoids，it
shows good feasibility. |
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