文章摘要
赵美娜,赵早亚,孙彩云,李文笙.投喂模式对吉富罗非鱼摄食和生长的影响[J].广东农业科学,2017,44(3):120-127
查看全文    HTML 投喂模式对吉富罗非鱼摄食和生长的影响
Effects of feeding regime on food intake and growth ofnile tilapia( Oreochromis niloticus)
  
DOI:10.16768/j.issn.1004-874X.2017.03.018
中文关键词: 吉富罗非鱼  投喂模式  生长摄食  生化成分  mRNA 转录水平
英文关键词: nile tilapia  feeding regime  growth and food intake  biochemical composition  mRNA expression
基金项目:国家现代农业产业技术体系专项(CARS-49);国家现代农业人才支撑计划项目(2016-2020); 广东省海洋与渔业局海港建设和渔业产业专项(A201601C02);深圳市战略性新兴产业发展专项资金( NYSW 20140401010064)
作者单位
赵美娜,赵早亚,孙彩云,李文笙 有害生物控制与资源利用国家重点实验室/ 广东省水生经济动物良种繁育重点实验室/ 中山大学深圳研究院/ 中山大学生命科学学院广东 广州 510006 
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中文摘要:
      以不同的投喂水平(100%、90%、80%、70%、60%、50% 饱食投喂量)、每天投喂两次,不同 的投喂频率(每天分别投喂1、2、3、4 次)但日投喂总量(100% 饱食投喂量)相同、分别投喂吉富罗非 鱼幼鱼8 周,分析不同投喂水平和投喂频率对吉富罗非鱼摄食生长性能、白肌生化组成及相关摄食因子 mRNA 转录水平的影响。结果表明,投喂水平较高或较低均会导致饲料系数和下丘脑NPY mRNA 转录水平 升高,特定生长率、饲料转化率和CCK1 mRNA 转录水平降低,白肌生化成分中只有粗脂肪含量受投喂水 平的影响达到显著性差异;投喂频率对罗非鱼生长各指标的影响均未达到显著性差异,但可观察到各指标 的变化趋势,投喂频率过高或过低均会导致吉富罗非鱼的饲料转化率、特定生长率降低,饲料系数、下丘 脑NPY mRNA 转录水平升高,白肌各种生化成分含量随投喂频率的增加均有先升后降的趋势,全脑CCK1 mRNA 转录水平则逐渐降低,全脑Orexin mRNA 转录水平逐渐升高。综合分析各种指标,吉富罗非鱼的最 适投喂水平为80% 饱食投喂量,每天投喂两次。
英文摘要:
      In order to investigate the effects of feeding regime on growth and food intake of genetic improvement of farmed tilapia( GITF tilapia),an 8-week experiment was conducted to determine the optimal feeding level and feeding frequency for the aquaculture of tilapia. The study investigated the effects of different feeding levels( 100%, 90%,80%,70%,60%,50% of satiation diet) and feeding frequencies( once,twice,thrice,four times a day) on food intake,growth performance,biochemical composition of white muscle and mRNA expression level of CCK1, NPY,and Orexin in tilapia. Results showed that higher or lower feeding level always enhanced feed coefficient( FCE) and mRNA expression level of NPY in hypothalamus. On the contrary,specific growth rate( SGR),feed conversion ratio( FCR) and mRNA expression level of CCK1 showed signs of decrease. Only crud fat content displayed a positive response to ration level. Moreover,feeding frequency showed no significant effects on all indices. Higher or lower feeding frequency led to lower FCR and SGR. Increased feeding frequency negatively affected crud protein and lipid deposition in skeletal muscle. The mRNA expression level of CCK1 was lower by higher feeding frequency,while the mRNA expression level of Orexin was reversed. In summary,80% of satiation diet as optimal feeding level and feeding tilapia twice a day was proposed.
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