| 曾令达1，2，陆飞莹1，宋冠华1，2，曾海泉3.丁香酚、小檗碱及苦参碱对荔枝霜疫霉的抑制作用[J].广东农业科学,2017,44(5):106-111 |
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丁香酚、小檗碱及苦参碱对荔枝霜疫霉的抑制作用 |
| Inhibitory effects of eugenol，berberine and matrine on Peronophythora litchi |
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| DOI：10.16768/j.issn.1004-874X.2017.05.017 |
| 中文关键词: 丁香酚 小檗碱 苦参碱 荔枝霜疫霉 抑菌效果 |
| 英文关键词: eugenol berberine matrine Peronophythora litchi Chen inhibitory effect |
| 基金项目:广东省科技计划项目（2013B020304010）；惠州市科技计划项目（2009B010001024）；惠州学院自然
科学项目（C211·0220） |
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| 中文摘要: |
| 为了筛选防治荔枝霜疫霉（Peronophythora litchi Chen）的植物源抑菌活性物质，以丁香酚、小
檗碱及苦参碱3 种植物源抑菌活性物质为材料，采用菌丝生长速率法测定3 种材料抑制荔枝霜疫霉效果；
霜疫霉孢子囊接种荔枝果实后测定3 种物质在荔枝贮藏中的抑菌效果。结果表明，丁香酚、小檗碱、苦参碱
3 种植物提取物对荔枝霜疫霉均具有抑菌活性，其中丁香酚的毒力方程为y =1.5909x+9.6688，抑菌中浓度
（EC50）为1.16 μg/mL；小檗碱的毒力方程为y =1.2129x+6.6709，EC50 为42.03 μg/mL；苦参碱的毒力方程
为y =1.6217x+6.5849，EC50 为105.01 μg/mL；丁香酚处理接种霜疫霉的果实褐变指数为96.9%，病情指数
为100.0%，小檗碱和苦参碱处理褐变指数与对照差异不显著，但病情指数分别为68.4% 和75.3%，与对照
差异显著。3 种物质中丁香酚的EC50 最小，离体抑菌效果最强，其次为小檗碱和苦参碱；果实抑菌效果中最
佳为小檗碱，其次为苦参碱和丁香酚。 |
| 英文摘要: |
| In order to find botanical antifungal substances that can prevent Peronophythora litchi Chen，using
the mycelial growth rate in vitro culture inhibition test，the antifungal effects of eugenol，matrine and berberine were
measured after inoculation of litchi fruit in storage. The results show that three plant extracts had inhibitory effects on
Peronophythora. The toxicity regression equation of eugenol was y =1.5909x+9.6688，the EC50 was 1.16 μg/mL. The
toxicity regression equation of berberine was y =1.2129x+6.6709，the EC50 was 42.03 μg/mL. The toxicity regression
equation of matrine was y =1.6217x+6.5849，the EC50 was 105.06 μg/mL. The browning index of fruit inoculated
Phytophthora treated with eugenol was 96.9%，and the disease index was 100.0%. Compared with the control，the
browning index of berberine and matrine was not obvious，but the disease indexes were 68.4% and 75.3%，which
were significantly different from that of the control. Among the three plant extracts，eugenol had the best inhibitory
effect，next one was berberine and the worst was matrine. For inhibitory effect of fruit，the best was berberine，
followed by matrine and eugenol. |
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