| 于 波1，朱 玉2，袁 霖3，黄丽丽1，张佩霞1，邹春萍1，孙映波1.金花茶花丝愈伤组织培养及其对抗生素敏感性研究[J].广东农业科学,2017,44(11):38-43 |
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金花茶花丝愈伤组织培养及其对抗生素敏感性研究 |
| Calli tissue induction for Camellia nitidissima anther filaments and its sensitivity to antibiotics |
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| DOI：10.16768/j.issn.1004-874X.2017.11.007 |
| 中文关键词: 金花茶 花丝 愈伤组织培养 抗生素 敏感性 |
| 英文关键词: Camellia nitidissima anther filament calli cultivation antibiotics sensitivity |
| 基金项目:广州市科技计划项目（201604020031）；广东省公益研究与能力建设专项（2014B070706016）；广东
省现代农业科技创新联盟建设项目（2016LM3169）；农业部华南都市农业重点实验室开放课题（2014hhlm006） |
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| 中文摘要: |
| 利用金花茶幼嫩花蕾进行体外培养，建立了花丝愈伤组织培养体系。试验结果表明：外植体
表面消毒70% 乙醇浸泡的适宜时间为10 min；花丝愈伤组织诱导和增殖培养的适宜培养基为MS+TDZ 0.5
mg/L + 2，4-D 1.0 mg/L + 30 g/L 蔗糖（pH 5.8），培养条件为25（±1）℃黑暗。卡那霉素和潮霉素对愈伤组
织诱导和增殖培养的影响结果表明，卡那霉素100 mg/L 或潮霉素30 mg/L 导致花丝外植体全部褐化死亡；
卡那霉素75 mg/L 或潮霉素20 mg/L 能抑制愈伤组织从花丝外植体上产生；卡那霉素100 mg/L 或潮霉素25
mg/L 则能完全抑制愈伤组织的增殖并导致最终褐化死亡。 |
| 英文摘要: |
| This study established calli tissue induction system of Camellia nitidissima through In vitro culture
from tender anther filaments of immature buds. The results showed that the suitable explant disinfection was soaked
in 70% ethyl alcohol for 10 min. The optimal mediums for the callus induction and its multiplication was Murashige
and Skoog（ MS） medium supplemented with 0.5 mg/L N-phenyl-N’-1，2，3-thiadiazol-5-ylurea（ TDZ），1.0
mg/L 2，4-Dichlorophenoxyacetic acid（ 2，4-D），and 30 g/L sucrose（ pH 5.8） in the dark at 25（±1）℃. And
the influences of kanamycin and hygromycin on calli induction and multiplication were studied. The results showed
that lethal doses of kanamycin and hygromycin to anther filament explants were 100 mg/L and 30 mg/L，respectively；
either 75 mg/L kanamycin or 20 mg/L hygromycin could inhibit the calli production from the explant of anther filaments；either 100 mg/L kanamycin or 25 mg/L hygromycin could inhibit proliferiation of calli. |
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