| 刘文俊1，2，邱金辉1，阳佑天3，黄运茂1，2，许丹宁1，2，田允波1，2.鹅Caspase-1 基因的克隆与序列分析[J].广东农业科学,2017,44(12):144-150 |
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鹅Caspase-1 基因的克隆与序列分析 |
| Cloning and sequence analysis of Caspase-1 gene in Chinese goose |
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| DOI：10.16768/j.issn.1004-874X.2017.12.022 |
| 中文关键词: 鹅 半胱氨酸蛋白酶-1 基因克隆 序列分析 同源性分析 分子进化 |
| 英文关键词: goose Caspase-1 gene cloning sequence analysis homology analysis molecular evolution |
| 基金项目:广东省科技计划项目（2017A020208069, 2016A020210129）；广东省大学生创新训练项目（仲教字
[2017]33 号）；广州市科技计划项目（201604020061） |
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| 摘要点击次数: 1682 |
| 全文下载次数: 560 |
| 中文摘要: |
| 为系统了解鹅炎症系统，用Trizol 法提取马岗鹅脾脏总RNA，采用兼并引物RT-PCR 方法克
隆了马岗鹅Caspase-1 基因并进行了系统的生物信息学分析。测序结果显示，go-Caspase-1 基因完整ORF
区长813 bp，编码270 个氨基酸（GenBank 序列号为MG463109）。同源性分析显示，go-Caspase-1 基因
序列与鸭、鸡等禽类的同源性较高，与其他物种的差异较大。蛋白质功能结构域分析显示，不同物种间
Caspase-1 蛋白的Caspase 结构域高度相似。蛋白三维结构预测比较显示，go-Caspase-1 蛋白空间结构与鸡
和人的Caspase-1 主体结构相似。 |
| 英文摘要: |
| In order to systematically understand the inflammatory system of goose，the total RNA was extracted
from the spleen of Magang goose by Trizol method. The caspase-1 gene of goose was cloned by RT-PCR and the
bioinformatics analysis was carried out. Sequencing results showed that the complete ORF region of the go-Caspase-1
gene was 813 bp，encoding 270 amino acids（ GenBank ID NO：MG463109）. Homology analysis showed that the
sequence of go-Caspase-1 was homologous to duck and chicken，and its differences with other species were relatively
large. Protein functional domain analysis showed that the number and distribution of Caspase-1 protein domains were
similar in different species. The three-dimensional structure of protein showed that the structure of go-Caspase-1 was
similar to that of chicken and human Caspase-1. |
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