| 韩吉思1，方小荣2，申礼凤2，李建波1，姚 茜1，杨 梅1.红心杉组培苗增殖培养基的优化[J].广东农业科学,2018,45(4):63-69 |
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红心杉组培苗增殖培养基的优化 |
| Optimization of culture medium for plantlets of Cunninghamia lanceolata（ Lamb.） Hook. in tissue cultureproliferation |
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| DOI：10.16768/j.issn.1004-874X.2018.04.010 |
| 中文关键词: 红心杉 组培 香蕉汁 TDZ 增殖系数 叶绿素 可溶性糖 |
| 英文关键词: Cunninghamia lanceolata（ Lamb.） Hook. tissue culture banana juice TDZ multiplication
coefficient chlorophyll soluble sugar |
| 基金项目:广西科学研究与技术开发计划项目（桂科能13278008） |
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| 中文摘要: |
| 以优良红心杉〔Cunninghamia lanceolata（Lamb.）Hook.〕为材料，采用L9（34）正交试验设
计，研究基本培养基、细胞分裂素苯基噻二唑基脲（TDZ）及香蕉汁浓度对其组培苗增殖系数、叶绿素含
量、可溶性糖含量的影响。结果表明：对红心杉组培苗增殖系数的影响大小表现为基本培养基＞香蕉汁浓
度＞ TDZ，对叶绿素和可溶性糖含量的影响大小表现为基本培养基＞ TDZ ＞香蕉汁浓度。经坐标综合评定
发现，3/4MS+ 香蕉汁100 g/L 为红心杉组培苗增殖培养的最佳组合，增殖系数可达2.45、叶绿素含量达2.45
mg/g、可溶性糖含量达33.47 mg/g。 |
| 英文摘要: |
| Using Cunninghamia lanceolata（ Lamb.） Hook as material，through L9（34） orthogonal test
design，the effects of MS medium，TDZ concentration and banana juice concentration on the proliferation
coefficient，chlorophyll content and soluble sugar content of plantlets were studied. The results showed that：The
effects on the multiplication coefficient of the plantlets in tissue culture were as follows：basic medium > banana juice
concentration > TDZ concentration. The effect on the content of chlorophyll and soluble sugar was as follows：basic
medium > TDZ concentration > banana juice concentration. It is found by comprehensive evaluation of coordinates
that，3/4MS + banana juice 100 g/L was the best combination for the multiplication and culture of the plantlets in
tissue culture. Under this condition，the proliferationg coefficient was 2.45，the chlorophyll content was 2.45 mg/g，
the soluble sugar content was 33.47 mg/g. |
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