| 叶玉妍，陈 晓，杨礼香.土人参Actin 基因片段的克隆及序列分析[J].广东农业科学,2018,45(6):19-24 |
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土人参Actin 基因片段的克隆及序列分析 |
| Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn |
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| DOI：10.16768/j.issn.1004-874X.2018.06.004 |
| 中文关键词: 土人参 肌动蛋白 Actin 基因 克隆 序列分析 |
| 英文关键词: Talinum paniculatum Actin Actin gene clone sequence analysis |
| 基金项目:国家自然科学基金（31570257） |
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| 中文摘要: |
| 为土人参功能基因的表达与调控提供内参基因，根据已知植物Actin 基因的保守区设计简并 性
引物，采用RT-PCR 方法扩增得到土人参的Actin 基因片段，将该片段连接于pGEM-T 载体，测序获得一段
大小为598 bp 的基因片段，该片段编码198 个氨基酸。通过生物信息学软件分析，该序列与其他植物Actin
基因的cDNA 序列同源性均在85% 以上，氨基酸序列的同源性达到86% 以上，表明试验克隆所得基因片段
为土人参Actin 基因片段。将该Actin 基因片段命名为TpActin1，并登陆在GenaBank，登录号为MH333039。 |
| 英文摘要: |
| In order to provide reference gene for studying the expression pattern of functional genes in Talinum
paniculatum，the cDNA fragment of Actin from Talinum paniculatum；was amplified by RT-PCR，by adopting
degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI
database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was
analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs，encoding
a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence
identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA
fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank
（accession number: MH33309）. |
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