| 李 亚，黄贝芳，陈月银，高 新，陈龙通，李锦鸿.椰心叶甲高毒力绿僵菌菌株筛选及分子鉴定[J].广东农业科学,2018,45(7):94-99 |
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椰心叶甲高毒力绿僵菌菌株筛选及分子鉴定 |
| Screening and molecular identification of high virulentMetarhizium strains against Brontispa longissima |
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| DOI：10.16768/j.issn.1004-874X.2018.07.015 |
| 中文关键词: 椰心叶甲 绿僵菌 筛选 致病力 分子鉴定 |
| 英文关键词: Brontispa longissima Metarhizium screening virulence molecular identification |
| 基金项目:广东海洋大学博士科研启动项目（R17060）；广东海洋大学大学生创新创业训练计划项目CXXL2018012） |
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| 中文摘要: |
| 为获得高毒力绿僵菌菌株用于防治棕榈科植物害虫椰心叶甲的菌剂，从椰心叶甲为害严重的地区采集椰心叶甲僵虫，分离到16株疑似绿僵菌菌株。测定上述菌株对椰心叶甲成虫和四龄幼虫的致病力，从中筛选2株毒力较高的菌株CM1601和HK1304。CM1601对成虫和四龄幼虫的LT50分别为4.1、3.9 d，HK1304为4.4、4.2 d。经进一步毒力试验发现，菌株CM1601和HK1304对椰心叶甲的毒性随着孢子浓度的增加而上升，当浓度分别上升到1.0×108、2.5×108 孢子/mL时，菌株对椰心叶甲致病力达到峰值。通过对菌株CM1601和HK1304的ITS 序列及系统发育树分析，鉴定两菌株分别为金龟子绿僵菌和金龟子绿僵菌蝗变种。研究筛选得到的高致病力绿僵菌菌株为椰心叶甲的生物防治提供了基础材料。 |
| 英文摘要: |
| To obtain the high virulent Metarhizium strains toward the pest Brontispa longissima, The dead B. longissima were collected from the areas where coconuts were severely damaged by B. longissima. Sixteen strains of Metarhizium species were further isolated. The virulence was measured with adults and 4th-instar larvae of B. longissima, and the high virulent strains CM1601 and HK1304 were screened. The LT50 values of CM1601 were 4.1 and 3.9 d to adults and 4thinstar larvae of B. longissima, and those of HK1304 were 4.4 and 4.2 d, respectively. Through the further virulence test, we found the virulence of CM1601 and HK1304 reached peak when concentration of conidia were 1.0×108 conidia/mL and 2.5×108 conidia/mL, respectively. The strain CM1601 and HK1304 were identified as M. anisopliae and M. anisopliae var. acridum by the analysis of ITS sequences and phylogenetic tree. Thus, both strains are highly potential for use in microbial control of B. longissima. |
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