| 宋郭柳 1
，王梦华 2，郑利锦 1
，袁峰均 3，史正军 3.深圳填海区植物根际土壤耐盐菌株筛选与鉴定[J].广东农业科学,2020,47(1):62-67 |
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深圳填海区植物根际土壤耐盐菌株筛选与鉴定 |
| Screening and Identification of Salt-tolerant Bacteria Strains in Rhizosphere Soils from Reclamation Area in Shenzhen |
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| DOI：10.16768/j.issn.1004-874X.2020.01.009 |
| 中文关键词: 耐盐菌 根际土壤 分子鉴定 碳源利用 |
| 英文关键词: salt-tolerant bacteria rhizosphere soil molecular identification carbon source utilization |
| 基金项目:深圳市科技计划技术攻关项目（JSGG20170824090848015）；深圳市城管科研项目（201915） |
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| 中文摘要: |
| 【目的】耐盐菌在盐渍土壤中分布广泛，研究其分布特征可为下一步盐渍土改良和降低植物盐害
工作提供有益参考。【方法】从深圳坝光和宝安填海区灌木根际采集 10 个土壤样品，利用不同浓度的 NaCl 培
养基分离耐盐菌种，并通过电镜形态观察和基因组 DNA 扩增测序对其进行鉴定。【结果】从灌木根际土壤中分
离出 66 株根际菌株，其中 14 株具有强耐盐能力，可在 10%~14% NaCl 浓度下生长。电镜结果和 16S rDNA 序列
分析表明，深圳坝光和宝安填海区土壤耐盐菌种类丰富且形态差异明显，14 株细菌中有 13 株来自芽孢杆菌属
（Bacillus），1 株来自溶杆菌属（Lysobacter）。此外，对其中 3 株耐盐菌的碳源利用能力分析结果表明，3 株
菌具有不同的碳源利用能力，NY12 可利用的碳源种类最多。【结论】首次对灌木根际耐盐菌株进行分离鉴定，
共分离出 14 株强耐盐菌株，其形态和碳源利用能力差异显著。 |
| 英文摘要: |
| 【Objective】Salt-tolerant bacteria are widely distributed in saline soils，and the study of their distribution
characteristics can provide useful reference for further improving saline soil and reducing plant salt damage. 【Methods】
10 soil samples were collected from shrub rhizospheres of reclamation areas in Baguang and Bao' an, Shenzhen. Salt-tolerant
strains were isolated by NaCl media of different concentrations, and identified by electron morphologic observation and
genomic DNA amplification sequencing. 【Results】A total of 66 strains were isolated from shrub rhizosphere soil, of which
14 strains had strong salt-tolerance and could grow at 10%-14% NaCl concentration. The results of electron microscopy and
16S rDNA sequence analysis showed that salt-tolerant bacteria were abundant and different in morphology in reclamation
areas of Baguang and Bao'an, Shenzhen. Among the 14 bacteria, 13 strains were from Bacillus and 1 strain was from
Lysobacter. In addition, the analysis of carbon utilization capacity of 3 salt-tolerant bacteria showed that the 3 bacteria
had different carbon utilization capacity, and NY12 had the most available carbon sources. 【Conclusion】14 strains with
strong salt-tolerance were isolated from the shrub rhizospheres for the first time, and their morphology and carbon utilizationcapacity were significantly different. |
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