| 叶远俊 1，谭健俊 1，李冬梅 1，刘金梅 1，柯 帅 2，郑夏华 2，徐晔春 1.激素和热水处理对姜荷花种子发芽的影响[J].广东农业科学,2020,47(10):41-48 |
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激素和热水处理对姜荷花种子发芽的影响 |
| Effects of Hormones and Hot Water Treatment on Germination of Curcuma alismatifolia Seed |
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| DOI：10.16768/j.issn.1004-874X.2020.10.006 |
| 中文关键词: 姜荷花 育种周期 浸种处理 发芽率 解除休眠 |
| 英文关键词: Curcuma alismatifolia breeding period soaking treatment germination rate dormancy release |
| 基金项目:广东省重点领域研发计划项目（2020B020220007） |
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| 摘要点击次数: 1143 |
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| 中文摘要: |
| 【目的】以姜荷花杂交种子为研究对象，通过不同浸种处理试验，打破姜荷花种子休眠，缩短姜荷花育种周期。【方法】利用激素、热水组合处理的方式，设置不同的浓度和浸种时间，以清水为对照，在 28 ℃条件下采用培养皿滤纸法催芽 60 d，统计发芽势和发芽率。【结果】与对照相比，外源激素和热水处理均能有效打破姜荷花种子休眠，使其提前 1 个月开始发芽。外源激素浸种试验中，400 mg/L GA3 浸种处理效果最好，发芽势和发芽率分别为 29.3%、50.7%；80 ℃热水浸种 5 min 效果最好，发芽势和发芽率分别为 10.3%、17%，但部分种子出现烫死现象；在组合浸种处理试验中，60 ℃热水浸种 10 min + 400 mg/L GA3 浸种 24 h 为最佳的处理组合，发芽势为 40.7%，播种 60 d 发芽率达到 64.3%。【结论】激素和热水组合处理对姜荷花种子解除休眠的效果显著优于其他处理，浸种后种子发芽提前，出苗更加整齐，适合育种实践中推广应用。 |
| 英文摘要: |
| 【Objective】In order to break the dormancy and shorten the breeding period, the seeds of Curcuma alismatifolia‘Spring’×C. alismatifolia‘Scarlet’were soaked under different treatments.【Method】The experiment was conducted by using three treating methods including hormones, hot water and their combination. The soaking time and hormone concentration were different for each group. All the seeds were pregerminated for two months by using filter paper method at 28 ℃ . The germination potential and germination rate of the control group（clean water）and experimental group were investigated.【Result】Compared with the control group, the seeds of experimental groups were germinated one month in advance by using hormones and hot water treatment. It was found that the germination potential and germination rate exhibited highest values of 29.3% and 50.7% respectively under 400 mg/L GA3 treatment. Hot water soaking for five minutes at 80 ℃ reached an optimal effect, with the germination potential and germination rate of 10.3% and 17%, respectively. However, part of the seeds suffered death from scald. Moreover, it was regarded as the perfect solution through combining hot water soaking for 10 minutes at 60 ℃ with 400 mg/L GA3 treatment for 24 h. The germination potential was 40.7% and the germination rate was up to 64.3% after sowing two months.【Conclusion】The effects of combination of hormones and hot water treatment were significantly better than other treatments for the seeds germination of C. alismatifolia. This method was suitable to be applied in breeding practices due to its guarantee of early germination and uniform seedling. |
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