| 陈 锐，门 欣，瞿 佳，孙晓宇，赵玲侠.微 生物菌剂 W55 修复多菌灵污染土壤研究[J].广东农业科学,2021,48(7):92-102 |
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微 生物菌剂 W55 修复多菌灵污染土壤研究 |
| Study on Remediation of Carbendazim-contaminated Soil by Microbial Agent W55 |
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| DOI：10.16768/j.issn.1004-874X.2021.07.012 |
| 中文关键词: 真菌 多菌灵 链格孢 生物降解 污染修复 |
| 英文关键词: fungi carbendazim Alternaria biodegradation pollution remediation |
| 基金项目:陕西省科学院科技计划项目（2018K-08，2018NK-01） |
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| 中文摘要: |
| 【目的】评估多菌灵降解菌致密链格孢 WJD-55 在棚室土壤中的应用效果及其安全性。【方法】
在设施棚室土壤中添加多菌灵并施用由降解菌 WJD-55 制备的菌剂 W55，栽种番茄作为观测作物，进行为期 50
d 的土壤修复试验，同时监测土壤中残留多菌灵、菌剂微生物 WJD-55 定殖及土壤真菌群落的变化，以及土壤
理化性质变化，观测作物生长等指标，从而评估菌剂 W55 在棚室土壤施用效果及其安全性。【 结果】初始土
壤中多菌灵质量浓度约 42 mg/kg，菌剂使用量 0.1%，药后第 10 天，土壤中多菌灵质量浓度为 9.27 mg/kg、下降
78%，而对照土壤中多菌灵质量浓度为 30.2 mg/kg、下降 29%，差异极显著（T-test，P=0.001）；土壤微生物高
通量测序结果显示，降解菌 WJD-55 可在污染土壤中定殖，弥补多菌灵对土壤微生物多样性产生的负面影响；
土壤理化指标显示，菌剂的加入未对土壤物理化学性质带来显著影响；观测作物番茄生长未受到菌剂的影响，
降解菌 WJD-55 未对番茄叶片等造成病害。【结论】多菌灵降 解菌致密链格孢 WJD-55 可在土壤中稳定定殖并
有效发挥降解多菌灵的作用，迅速降低土壤残留多菌灵含量，可改善污染土壤真菌微生物多样性，对土壤理化
性质及番茄作物生长无不良影响。 |
| 英文摘要: |
| 【Objective】The study was carried out to verify the application effect and safety of carbendazim
degrading strain Alternaria compacta WJD-55 in greenhouse soil.【Method】Carbendazim was added to the greenhouse
soil and microbial agent W55 made with WJD-55 were applied at same time, and tomatoes were cultivated for observation.
The changes of the remaining carbendazim, colonization of WJD-55 and soil fungal community, soil physical and chemical
properties and planting growth of tomatoes were monitored during 50 days of remediation, with a view to evaluate the
application effect of microbial agent W55 and its safety.【Result】The carbendazim content in initial soil about 42 mg/kg
and the application amount of the agent was 0.1%. After treatment for 10 days, carbendazim residual was 9.27 mg/kg, with
a decrease of 78% . While in control soil, carbendazim residual was about 30.2 mg/kg, with a decrease of 29%. There was
extremely significant difference（T-test, P=0.001）between two groups. High throughput sequencing of soil microorganism
showed that WJD-55 could colonize in soil stably, which rescued the negative impacts of carbendazim on microbial diversity.
The physical and chemical indexes of soil showed the microbial agent W55 had no significant effect on soil physical and chemical properties. The growth of tomato plant was not affected by the agent and WJD-55 showed no harm to the tomato
leaves.【Conclusion】Alternaria compacta strain WJD-55 could colonize stably in soil and degrade carbendazim effectively,
decrease the carbendazim content in soil rapidly, improve the fungal microorganism diversity of the contaminated soil, and has
no negative effect on soil and plant. |
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