文章摘要
颜妙珍,李兰兰,冯名开,崔芸瑜,陈棕林,周岐伟,赖家业.不同处理方法对百香果种子萌发的影响[J].广东农业科学,2022,49(2):37-44
查看全文    HTML 不同处理方法对百香果种子萌发的影响
Effects of Different Treatments on SeedGermination of Passion Fruit
  
DOI:10.16768/j.issn.1004-874X.2022.02.005
中文关键词: 百香果  种子萌发  发芽率  发芽指数  发芽势
英文关键词: passion fruit  seed germination  germination percentage  germination index  germination potential
基金项目:广西科技重大专项(桂科 AA17204058-17);广西创新驱动发展专项(17204058)
作者单位
颜妙珍,李兰兰,冯名开,崔芸瑜,陈棕林,周岐伟,赖家业  
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中文摘要:
      【目的】研究百香果种子的萌发特性,为百香果种子育苗及杂交后代种子萌发、性状观察奠定基础。 【方法】以百香果种子为试验材料,研究不同水温(25、45、65、85、100 ℃)、浓硫酸浸泡时间(0、0.5、1、 2、5、8、10、15 min)、赤霉素浓度(0、200、400、800 mg/L)、培养温度(18、28、35、18~28、28~35 ℃)、 有无种壳及不同物理处理方式(针刺、磨砂)对百香果种子萌发的影响,以发芽率、发芽势、发芽指数为考查 指标,确定种子萌发适宜条件。【结果】百香果种子在 65 ℃温水中自然冷却浸泡 24 h 发芽率最高(57.78%); 浓硫酸最适浸泡时间为 8 min,发芽率、发芽势和发芽指数分别为 77.38%、77.38% 和 3.39;赤霉素浓度为 400 mg/L 时发芽率高达 81.11%;最适发芽培养温度为 28~35 ℃变温,发芽率为 64.44%;全剥壳的百香果种子发 芽率达 76.67%;磨砂和针刺处理对种子萌发的影响差异不显著,但针刺不好控制容易伤到种胚,以磨砂为佳, 发芽率为 60%。【结论】百香果种子适宜用 65 ℃温水自然冷却浸泡 24 h 或浓硫酸处理 8 min 后浸泡 24 h,采用 磨砂处理或 400 mg/L 赤霉素浸泡 24 h 可有效提高种子发芽率,适宜的萌发培养温度为 28~35 ℃变温。
英文摘要:
      【Objective】The study was performed to explore the germination characteristics of Passiflora edulia Sims seeds in order to lay a foundation for seed seedling raising and seed germination and character observation of hybrid offspring of P. edulia.【Method】P. edulia seeds were used as experimental materials to study the effects of different water temperatures (25, 45, 65, 85, 100 ℃ ), time of soaking in concentrated sulfuric acid (0, 0.5, 1, 2, 5, 8, 10 and 15 min), gibberellin concentrations (0, 200, 400 and 800 mg/L), culture temperatures (18, 28, 35, 18-28, 28-35 ℃ ), seed shells and different physical treatment methods (acupuncture and sanding) on seed germination of P. edulia. Taking germination rate, germination potential and germination index as test indexes, different germination conditions were evaluated to determine the suitable ones for seed germination.【Result】The germination rate of P. edulia seeds soaked in 65℃ warm water for 24 h was the highest (57.78%). The optimum soaking time in concentrated sulfuric acid was 8 min, the germination rate, germination potential and germination index were 77.38%, 77.38% and 3.39%, respectively. The germination rate was 81.11% when the soaking concentration of gibberellin was 400 mg/L. The optimum culture temperature for germination was 28-35℃ , and the germination rate was 64.44%. The seed germination rate of P. edulia was 76.67%. There was no significant difference in seed germination between physical treatment and acupuncture treatment, but acupuncture was not well controlled and easy to hurt seed embryo. Grinding treatment was the best, and the germination rate was 60%. 【Conclusion】The seeds of P. edulia are suitable to be soaked in 65℃ warm water for 24 h or soaked in concentrated sulfuric acid for 8 min for 24 h; The seed germination rate can be effectively improved by grinding treatment or soaking in 400 mg/L gibberellin for 24 h. The suitable germination culture temperature is 28-35℃ .
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